Understanding Biological Regulation Through Synthetic Biology
Engineering synthetic gene regulatory circuits proceeds through iterative cycles of design, building, and testing. Initial circuit designs must rely on often-incomplete models of regulation established by fields of reductive inquiry—biochemistry and molecular and systems biology. As differences in d...
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| Format: | Article |
| Language: | en_US |
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Annual Reviews
2018
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| Online Access: | http://hdl.handle.net/1721.1/119222 https://orcid.org/0000-0002-5560-8246 |
| _version_ | 1811095814721765376 |
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| author | Bashor, Caleb Collins, James J. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Bashor, Caleb Collins, James J. |
| author_sort | Bashor, Caleb |
| collection | MIT |
| description | Engineering synthetic gene regulatory circuits proceeds through iterative cycles of design, building, and testing. Initial circuit designs must rely on often-incomplete models of regulation established by fields of reductive inquiry—biochemistry and molecular and systems biology. As differences in designed and experimentally observed circuit behavior are inevitably encountered, investigated, and resolved, each turn of the engineering cycle can force a resynthesis in understanding of natural network function. Here, we outline research that uses the process of gene circuit engineering to advance biological discovery. Synthetic gene circuit engineering research has not only refined our understanding of cellular regulation but furnished biologists with a toolkit that can be directed at natural systems to exact precision manipulation of network structure. As we discuss, using circuit engineering to predictively reorganize, rewire, and reconstruct cellular regulation serves as the ultimate means of testing and understanding how cellular phenotype emerges from systems-level network function. Keywords: synthetic biology; regulatory network; synthetic gene circuit; engineering cycle; motif; refactoring |
| first_indexed | 2024-09-23T16:29:06Z |
| format | Article |
| id | mit-1721.1/119222 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:29:06Z |
| publishDate | 2018 |
| publisher | Annual Reviews |
| record_format | dspace |
| spelling | mit-1721.1/1192222022-09-29T19:58:31Z Understanding Biological Regulation Through Synthetic Biology Understanding Biological Regulation Through Synthetic Biology Bashor, Caleb Collins, James J. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Synthetic Biology Center Collins, James J Bashor, Caleb Collins, James J. Engineering synthetic gene regulatory circuits proceeds through iterative cycles of design, building, and testing. Initial circuit designs must rely on often-incomplete models of regulation established by fields of reductive inquiry—biochemistry and molecular and systems biology. As differences in designed and experimentally observed circuit behavior are inevitably encountered, investigated, and resolved, each turn of the engineering cycle can force a resynthesis in understanding of natural network function. Here, we outline research that uses the process of gene circuit engineering to advance biological discovery. Synthetic gene circuit engineering research has not only refined our understanding of cellular regulation but furnished biologists with a toolkit that can be directed at natural systems to exact precision manipulation of network structure. As we discuss, using circuit engineering to predictively reorganize, rewire, and reconstruct cellular regulation serves as the ultimate means of testing and understanding how cellular phenotype emerges from systems-level network function. Keywords: synthetic biology; regulatory network; synthetic gene circuit; engineering cycle; motif; refactoring 2018-11-20T15:12:07Z 2018-11-20T15:12:07Z 2018-05 2018-03 Article http://purl.org/eprint/type/JournalArticle 1936-122X 1936-1238 http://hdl.handle.net/1721.1/119222 Bashor, Caleb J. and James J. Collins. “Understanding Biological Regulation Through Synthetic Biology.” Annual Review of Biophysics 47, 1 (May 2018): 399–423 © 2018 Annual Review https://orcid.org/0000-0002-5560-8246 en_US https://doi.org/10.1146/annurev-biophys-070816-033903 Annual Review of Biophysics Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Annual Reviews Prof. Collins via Howard Silver |
| spellingShingle | Bashor, Caleb Collins, James J. Understanding Biological Regulation Through Synthetic Biology |
| title | Understanding Biological Regulation Through Synthetic Biology |
| title_full | Understanding Biological Regulation Through Synthetic Biology |
| title_fullStr | Understanding Biological Regulation Through Synthetic Biology |
| title_full_unstemmed | Understanding Biological Regulation Through Synthetic Biology |
| title_short | Understanding Biological Regulation Through Synthetic Biology |
| title_sort | understanding biological regulation through synthetic biology |
| url | http://hdl.handle.net/1721.1/119222 https://orcid.org/0000-0002-5560-8246 |
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