Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism
We present the design, analysis, and validation of a tunable-volume handheld pipette that enables precise drawing and dispensing of ml and μl liquid volumes. The design builds upon the standard mechanism of a handheld micropipette by incorporating an elastic diaphragm that de-amplifies the volume di...
| Main Authors: | , , , |
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| Format: | Article |
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ASME International
2018
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| Online Access: | http://hdl.handle.net/1721.1/119377 https://orcid.org/0000-0002-9448-6894 https://orcid.org/0000-0001-9666-1732 https://orcid.org/0000-0001-5409-3722 https://orcid.org/0000-0002-7372-3512 |
| _version_ | 1811080816638296064 |
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| author | Beroz, Justin Douglas Jiang, Sheng Lewandowski, John Robert Hart, Anastasios John |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Beroz, Justin Douglas Jiang, Sheng Lewandowski, John Robert Hart, Anastasios John |
| author_sort | Beroz, Justin Douglas |
| collection | MIT |
| description | We present the design, analysis, and validation of a tunable-volume handheld pipette that enables precise drawing and dispensing of ml and μl liquid volumes. The design builds upon the standard mechanism of a handheld micropipette by incorporating an elastic diaphragm that de-amplifies the volume displacement of the internal piston via compression of an entrapped air volume. The degree of de-amplification is determined by the stiffness of the elastic diaphragm and the amount of entrapped air. An analytical model of the diaphragm mechanism is derived, which guides how to achieve linear deamplification over an extended range where leading-order nonlinear contributions are significant. In particular, nonlinearities inherent in the mechanical behavior of the diaphragm and entrapped air volume may exactly cancel one another by careful design of the pipette's parameter constants. This linearity is a key attribute for enabling the pipette's tunable volumetric range, as this allows diaphragms with different stiffnesses to be selectively used with a conventional linearstepping piston mechanism. Design considerations regarding the range, accuracy, and precision of the proposed pipette are detailed based on the model. Additionally, we have constructed a handheld prototype that uses a planar latex sheet as the diaphragm. Our pipetting experiments validate the derived model and exhibit linearity between the piston stroke and drawn liquid volume. We propose that this design enables a single handheld mechanical pipette to achieve drawing and dispensing of liquids over a 1μl-10ml range (i.e., the range of the entire micropipette suite), with volumetric resolution and precision comparable to commercially available counterparts. |
| first_indexed | 2024-09-23T11:37:20Z |
| format | Article |
| id | mit-1721.1/119377 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:37:20Z |
| publishDate | 2018 |
| publisher | ASME International |
| record_format | dspace |
| spelling | mit-1721.1/1193772022-10-01T04:51:14Z Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism Beroz, Justin Douglas Jiang, Sheng Lewandowski, John Robert Hart, Anastasios John Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Physics Beroz, Justin Douglas Jiang, Sheng Lewandowski, John Robert Hart, Anastasios John We present the design, analysis, and validation of a tunable-volume handheld pipette that enables precise drawing and dispensing of ml and μl liquid volumes. The design builds upon the standard mechanism of a handheld micropipette by incorporating an elastic diaphragm that de-amplifies the volume displacement of the internal piston via compression of an entrapped air volume. The degree of de-amplification is determined by the stiffness of the elastic diaphragm and the amount of entrapped air. An analytical model of the diaphragm mechanism is derived, which guides how to achieve linear deamplification over an extended range where leading-order nonlinear contributions are significant. In particular, nonlinearities inherent in the mechanical behavior of the diaphragm and entrapped air volume may exactly cancel one another by careful design of the pipette's parameter constants. This linearity is a key attribute for enabling the pipette's tunable volumetric range, as this allows diaphragms with different stiffnesses to be selectively used with a conventional linearstepping piston mechanism. Design considerations regarding the range, accuracy, and precision of the proposed pipette are detailed based on the model. Additionally, we have constructed a handheld prototype that uses a planar latex sheet as the diaphragm. Our pipetting experiments validate the derived model and exhibit linearity between the piston stroke and drawn liquid volume. We propose that this design enables a single handheld mechanical pipette to achieve drawing and dispensing of liquids over a 1μl-10ml range (i.e., the range of the entire micropipette suite), with volumetric resolution and precision comparable to commercially available counterparts. National Science Foundation (U.S.). Graduate Research Fellowship National Defense Science and Engineering Graduate (NDSEG) Fellowship National Science Foundation (U.S.). Career Award (CMMI -1150585) National Science Foundation (U.S.). Career Award (CMMI -1346638) Massachusetts Institute of Technology. Department of Mechanical Engineering (startup funds) 2018-12-03T13:10:57Z 2018-12-03T13:10:57Z 2014-08 2018-11-29T17:41:47Z Article http://purl.org/eprint/type/ConferencePaper 978-0-7918-4637-7 http://hdl.handle.net/1721.1/119377 Beroz, Justin, Sheng Jiang, John Lewandowski, and A. John Hart. “Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism.” Volume 5B: 38th Mechanisms and Robotics Conference (August 17, 2014). https://orcid.org/0000-0002-9448-6894 https://orcid.org/0000-0001-9666-1732 https://orcid.org/0000-0001-5409-3722 https://orcid.org/0000-0002-7372-3512 http://dx.doi.org/10.1115/DETC2014-34758 Volume 5B: 38th Mechanisms and Robotics Conference Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf ASME International ASME |
| spellingShingle | Beroz, Justin Douglas Jiang, Sheng Lewandowski, John Robert Hart, Anastasios John Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism |
| title | Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism |
| title_full | Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism |
| title_fullStr | Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism |
| title_full_unstemmed | Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism |
| title_short | Tunable-Volume Handheld Pipette Utilizing a Pneumatic De-Amplification Mechanism |
| title_sort | tunable volume handheld pipette utilizing a pneumatic de amplification mechanism |
| url | http://hdl.handle.net/1721.1/119377 https://orcid.org/0000-0002-9448-6894 https://orcid.org/0000-0001-9666-1732 https://orcid.org/0000-0001-5409-3722 https://orcid.org/0000-0002-7372-3512 |
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