Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays
Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an...
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| Format: | Article |
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Transducers Research Foundation, Inc.
2018
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| Online Access: | http://hdl.handle.net/1721.1/119378 https://orcid.org/0000-0002-7372-3512 |
| _version_ | 1826208264777367552 |
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| author | Chen, Yu-Chih Baac, Hyoung Won Lee, Kyu-Tae Teichert, Kendall Guo, L. Jay Yoon, Euisik Hart, Anastasios John |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Chen, Yu-Chih Baac, Hyoung Won Lee, Kyu-Tae Teichert, Kendall Guo, L. Jay Yoon, Euisik Hart, Anastasios John |
| author_sort | Chen, Yu-Chih |
| collection | MIT |
| description | Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells. |
| first_indexed | 2024-09-23T14:03:06Z |
| format | Article |
| id | mit-1721.1/119378 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:03:06Z |
| publishDate | 2018 |
| publisher | Transducers Research Foundation, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/1193782022-10-01T18:52:26Z Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays Chen, Yu-Chih Baac, Hyoung Won Lee, Kyu-Tae Teichert, Kendall Guo, L. Jay Yoon, Euisik Hart, Anastasios John Massachusetts Institute of Technology. Department of Mechanical Engineering Hart, Anastasios John Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells. United States. Department of Defense (W81XWH-12-1-0325) National Institutes of Health (U.S.) (1R21CA17585701) 2018-12-03T13:41:23Z 2018-12-03T13:41:23Z 2015-10 2018-11-29T17:53:15Z Article http://purl.org/eprint/type/ConferencePaper 1556-5890 1545-8288 http://hdl.handle.net/1721.1/119378 Chen, Yu-Chih, Hyoung Won Baac, Kyu-Tae Lee, Kendall Teichert, A. John Hart, L. Jay Guo and Euisik Yoon. "Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays." 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, October 25-29, 2015, Gyeongju, Korea. https://orcid.org/0000-0002-7372-3512 http://www.rsc.org/images/LOC/2015/PDFs/Papers/0224_3B2-2.pdf 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Transducers Research Foundation, Inc. Other repository |
| spellingShingle | Chen, Yu-Chih Baac, Hyoung Won Lee, Kyu-Tae Teichert, Kendall Guo, L. Jay Yoon, Euisik Hart, Anastasios John Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays |
| title | Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays |
| title_full | Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays |
| title_fullStr | Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays |
| title_full_unstemmed | Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays |
| title_short | Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays |
| title_sort | selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt coated microwell arrays |
| url | http://hdl.handle.net/1721.1/119378 https://orcid.org/0000-0002-7372-3512 |
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