YY1 Is a Structural Regulator of Enhancer-Promoter Loops
There is considerable evidence that chromosome structure plays important roles in gene control, but we have limited understanding of the proteins that contribute to structural interactions between gene promoters and their enhancer elements. Large DNA loops that encompass genes and their regulatory e...
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Elsevier BV
2018
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| Online Access: | http://hdl.handle.net/1721.1/119424 https://orcid.org/0000-0003-0998-9882 https://orcid.org/0000-0001-8091-6112 https://orcid.org/0000-0001-6263-2423 https://orcid.org/0000-0001-8855-8647 |
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| author | Sigova, Alla A. Hannett, Nancy M. Day, Daniel S. Abraham, Brian J. Cohen, Malkiel A. Nabet, Behnam Buckley, Dennis L. Guo, Yang Eric Hnisz, Denes Bradner, James E. Gray, Nathanael S. Weintraub, Abraham Selby Li, Charles Han Zamudio Montes de Oca, Alicia Jaenisch, Rudolf Young, Richard A. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Sigova, Alla A. Hannett, Nancy M. Day, Daniel S. Abraham, Brian J. Cohen, Malkiel A. Nabet, Behnam Buckley, Dennis L. Guo, Yang Eric Hnisz, Denes Bradner, James E. Gray, Nathanael S. Weintraub, Abraham Selby Li, Charles Han Zamudio Montes de Oca, Alicia Jaenisch, Rudolf Young, Richard A. |
| author_sort | Sigova, Alla A. |
| collection | MIT |
| description | There is considerable evidence that chromosome structure plays important roles in gene control, but we have limited understanding of the proteins that contribute to structural interactions between gene promoters and their enhancer elements. Large DNA loops that encompass genes and their regulatory elements depend on CTCF-CTCF interactions, but most enhancer-promoter interactions do not employ this structural protein. Here, we show that the ubiquitously expressed transcription factor Yin Yang 1 (YY1) contributes to enhancer-promoter structural interactions in a manner analogous to DNA interactions mediated by CTCF. YY1 binds to active enhancers and promoter-proximal elements and forms dimers that facilitate the interaction of these DNA elements. Deletion of YY1 binding sites or depletion of YY1 protein disrupts enhancer-promoter looping and gene expression. We propose that YY1-mediated enhancer-promoter interactions are a general feature of mammalian gene control. YY1 is a structural regulator of enhancer-promoter interactions and facilitates gene expression. |
| first_indexed | 2024-09-23T15:18:42Z |
| format | Article |
| id | mit-1721.1/119424 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:18:42Z |
| publishDate | 2018 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1194242022-10-02T02:05:37Z YY1 Is a Structural Regulator of Enhancer-Promoter Loops Sigova, Alla A. Hannett, Nancy M. Day, Daniel S. Abraham, Brian J. Cohen, Malkiel A. Nabet, Behnam Buckley, Dennis L. Guo, Yang Eric Hnisz, Denes Bradner, James E. Gray, Nathanael S. Weintraub, Abraham Selby Li, Charles Han Zamudio Montes de Oca, Alicia Jaenisch, Rudolf Young, Richard A. Massachusetts Institute of Technology. Department of Biology Weintraub, Abraham Selby Li, Charles Han Zamudio Montes de Oca, Alicia Jaenisch, Rudolf Young, Richard A There is considerable evidence that chromosome structure plays important roles in gene control, but we have limited understanding of the proteins that contribute to structural interactions between gene promoters and their enhancer elements. Large DNA loops that encompass genes and their regulatory elements depend on CTCF-CTCF interactions, but most enhancer-promoter interactions do not employ this structural protein. Here, we show that the ubiquitously expressed transcription factor Yin Yang 1 (YY1) contributes to enhancer-promoter structural interactions in a manner analogous to DNA interactions mediated by CTCF. YY1 binds to active enhancers and promoter-proximal elements and forms dimers that facilitate the interaction of these DNA elements. Deletion of YY1 binding sites or depletion of YY1 protein disrupts enhancer-promoter looping and gene expression. We propose that YY1-mediated enhancer-promoter interactions are a general feature of mammalian gene control. YY1 is a structural regulator of enhancer-promoter interactions and facilitates gene expression. National Institutes of Health (U.S.) (Grant HG002668/GM123511) National Institutes of Health (U.S.) (Grant R37HD045022/R01-NS088538/R01-MH104610) Virginia and Daniel K. Ludwig Graduate Fellowship National Science Foundation (U.S.). Graduate Research Fellowship Program 2018-12-04T18:36:36Z 2018-12-04T18:36:36Z 2017-12 2017-09 2018-12-04T14:13:23Z Article http://purl.org/eprint/type/JournalArticle 0092-8674 http://hdl.handle.net/1721.1/119424 Weintraub, Abraham S., Charles H. Li, Alicia V. Zamudio, Alla A. Sigova, Nancy M. Hannett, Daniel S. Day, Brian J. Abraham, et al. “YY1 Is a Structural Regulator of Enhancer-Promoter Loops.” Cell 171, no. 7 (December 2017): 1573–1588.e28. © 2017 Elsevier Inc. https://orcid.org/0000-0003-0998-9882 https://orcid.org/0000-0001-8091-6112 https://orcid.org/0000-0001-6263-2423 https://orcid.org/0000-0001-8855-8647 http://dx.doi.org/10.1016/J.CELL.2017.11.008 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Sigova, Alla A. Hannett, Nancy M. Day, Daniel S. Abraham, Brian J. Cohen, Malkiel A. Nabet, Behnam Buckley, Dennis L. Guo, Yang Eric Hnisz, Denes Bradner, James E. Gray, Nathanael S. Weintraub, Abraham Selby Li, Charles Han Zamudio Montes de Oca, Alicia Jaenisch, Rudolf Young, Richard A. YY1 Is a Structural Regulator of Enhancer-Promoter Loops |
| title | YY1 Is a Structural Regulator of Enhancer-Promoter Loops |
| title_full | YY1 Is a Structural Regulator of Enhancer-Promoter Loops |
| title_fullStr | YY1 Is a Structural Regulator of Enhancer-Promoter Loops |
| title_full_unstemmed | YY1 Is a Structural Regulator of Enhancer-Promoter Loops |
| title_short | YY1 Is a Structural Regulator of Enhancer-Promoter Loops |
| title_sort | yy1 is a structural regulator of enhancer promoter loops |
| url | http://hdl.handle.net/1721.1/119424 https://orcid.org/0000-0003-0998-9882 https://orcid.org/0000-0001-8091-6112 https://orcid.org/0000-0001-6263-2423 https://orcid.org/0000-0001-8855-8647 |
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