Quantitative phase microscopy of red blood cells during planar trapping and propulsion
Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling and deforming large cell populations along the length of the waveguide. Brigh...
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Royal Society of Chemistry
2019
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| Online Access: | http://hdl.handle.net/1721.1/119859 https://orcid.org/0000-0001-6880-9509 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826212045266092032 |
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| author | Ahmad, Azeem Dubey, Vishesh Singh, Vijay Raj Tinguely, Jean-Claude Øie, Cristina Ionica Wolfson, Deanna L. Mehta, Dalip Singh So, Peter T. C. Ahluwalia, Balpreet Singh |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ahmad, Azeem Dubey, Vishesh Singh, Vijay Raj Tinguely, Jean-Claude Øie, Cristina Ionica Wolfson, Deanna L. Mehta, Dalip Singh So, Peter T. C. Ahluwalia, Balpreet Singh |
| author_sort | Ahmad, Azeem |
| collection | MIT |
| description | Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling and deforming large cell populations along the length of the waveguide. Bright field microscopy employed with waveguide trapping does not provide quantitative information about structural changes. Here, we have combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation. By using interference microscopy, time-lapsed interferometric images of trapped RBCs were recorded in real-time and subsequently utilized to reconstruct optical phase maps. Quantification of the phase differences before and after trapping enabled study of the mechanical effects during planar trapping. During planar trapping, a decrease in the maximum phase values, an increase in the surface area and a decrease in the volume and sphericity of RBCs were observed. QPM was used to analyze the phase values for two specific regions within RBCs: the annular rim and the central donut. The phase value of the annular rim decreases whereas it increases for the central donut during planar trapping. These changes correspond to a redistribution of cytosol inside the RBC during planar trapping and transportation. |
| first_indexed | 2024-09-23T15:15:16Z |
| format | Article |
| id | mit-1721.1/119859 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:15:16Z |
| publishDate | 2019 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1198592022-09-29T13:41:49Z Quantitative phase microscopy of red blood cells during planar trapping and propulsion Ahmad, Azeem Dubey, Vishesh Singh, Vijay Raj Tinguely, Jean-Claude Øie, Cristina Ionica Wolfson, Deanna L. Mehta, Dalip Singh So, Peter T. C. Ahluwalia, Balpreet Singh Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Singh, Vijay Raj So, Peter T. C. Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling and deforming large cell populations along the length of the waveguide. Bright field microscopy employed with waveguide trapping does not provide quantitative information about structural changes. Here, we have combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation. By using interference microscopy, time-lapsed interferometric images of trapped RBCs were recorded in real-time and subsequently utilized to reconstruct optical phase maps. Quantification of the phase differences before and after trapping enabled study of the mechanical effects during planar trapping. During planar trapping, a decrease in the maximum phase values, an increase in the surface area and a decrease in the volume and sphericity of RBCs were observed. QPM was used to analyze the phase values for two specific regions within RBCs: the annular rim and the central donut. The phase value of the annular rim decreases whereas it increases for the central donut during planar trapping. These changes correspond to a redistribution of cytosol inside the RBC during planar trapping and transportation. Singapore-MIT Alliance for Research and Technology (SMART) 2019-01-04T18:47:58Z 2019-01-04T18:47:58Z 2018-08 2018-04 2019-01-04T14:28:27Z Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/119859 Ahmad, Azeem, Vishesh Dubey, Vijay Raj Singh, Jean-Claude Tinguely, Cristina Ionica Øie, Deanna L. Wolfson, Dalip Singh Mehta, Peter T. C. So, and Balpreet Singh Ahluwalia. “Quantitative Phase Microscopy of Red Blood Cells During Planar Trapping and Propulsion.” Lab on a Chip 18, no. 19 (2018): 3025–3036. © 2018 The Royal Society of Chemistry https://orcid.org/0000-0001-6880-9509 https://orcid.org/0000-0003-4698-6488 http://dx.doi.org/10.1039/c8lc00356d Lab on a Chip Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry (RSC) |
| spellingShingle | Ahmad, Azeem Dubey, Vishesh Singh, Vijay Raj Tinguely, Jean-Claude Øie, Cristina Ionica Wolfson, Deanna L. Mehta, Dalip Singh So, Peter T. C. Ahluwalia, Balpreet Singh Quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| title | Quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| title_full | Quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| title_fullStr | Quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| title_full_unstemmed | Quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| title_short | Quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| title_sort | quantitative phase microscopy of red blood cells during planar trapping and propulsion |
| url | http://hdl.handle.net/1721.1/119859 https://orcid.org/0000-0001-6880-9509 https://orcid.org/0000-0003-4698-6488 |
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