Digital micromirror device-based common-path quantitative phase imaging
We propose a novel common-path quantitative phase imaging (QPI) method based on a digital micromirror device (DMD). The DMD is placed in a plane conjugate to the objective back-aperture plane for the purpose of generating two plane waves thatilluminate the sample.Apinhole isused in the detection arm...
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| Format: | Article |
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The Optical Society
2019
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| Online Access: | http://hdl.handle.net/1721.1/120010 https://orcid.org/0000-0002-4761-6641 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1811097273860358144 |
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| author | Zheng, Cheng Zhou, Renjie Kuang, Cuifang Zhao, Guangyuan Yaqoob, Zahid So, Peter T. C. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Zheng, Cheng Zhou, Renjie Kuang, Cuifang Zhao, Guangyuan Yaqoob, Zahid So, Peter T. C. |
| author_sort | Zheng, Cheng |
| collection | MIT |
| description | We propose a novel common-path quantitative phase imaging (QPI) method based on a digital micromirror device (DMD). The DMD is placed in a plane conjugate to the objective back-aperture plane for the purpose of generating two plane waves thatilluminate the sample.Apinhole isused in the detection arm to filter one of the beams after sample to create a reference beam. Additionally, a transmission-type liquid crystal device, placed at the objective back-aperture plane, eliminates the specular reflection noise arising from all the "off" state DMD micromirrors, which is common in all DMD-based illuminations. We have demonstrated high sensitivity QPI, which has a measured spatial and temporal noise of 4.92 nm and 2.16 nm, respectively. Experiments with calibrated polystyrene beads illustrate the desired phase measurement accuracy. In addition, we have measured the dynamic height maps of red blood cell membrane fluctuations, showing the efficacy of the proposed system for live cell imaging. Most importantly, the DMD grants the system convenience in varying the interference fringe period on the camera to easily satisfy the pixel sampling conditions. This feature also alleviates the pinhole alignment complexity. We envision that the proposed DMD-based common-path QPI system will allow for system miniaturization and automation for a broader adaption. |
| first_indexed | 2024-09-23T16:57:04Z |
| format | Article |
| id | mit-1721.1/120010 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:57:04Z |
| publishDate | 2019 |
| publisher | The Optical Society |
| record_format | dspace |
| spelling | mit-1721.1/1200102022-10-03T09:22:49Z Digital micromirror device-based common-path quantitative phase imaging Zheng, Cheng Zhou, Renjie Kuang, Cuifang Zhao, Guangyuan Yaqoob, Zahid So, Peter T. C. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Laser Biomedical Research Center Zhou, Renjie Yaqoob, Zahid So, Peter T. C. We propose a novel common-path quantitative phase imaging (QPI) method based on a digital micromirror device (DMD). The DMD is placed in a plane conjugate to the objective back-aperture plane for the purpose of generating two plane waves thatilluminate the sample.Apinhole isused in the detection arm to filter one of the beams after sample to create a reference beam. Additionally, a transmission-type liquid crystal device, placed at the objective back-aperture plane, eliminates the specular reflection noise arising from all the "off" state DMD micromirrors, which is common in all DMD-based illuminations. We have demonstrated high sensitivity QPI, which has a measured spatial and temporal noise of 4.92 nm and 2.16 nm, respectively. Experiments with calibrated polystyrene beads illustrate the desired phase measurement accuracy. In addition, we have measured the dynamic height maps of red blood cell membrane fluctuations, showing the efficacy of the proposed system for live cell imaging. Most importantly, the DMD grants the system convenience in varying the interference fringe period on the camera to easily satisfy the pixel sampling conditions. This feature also alleviates the pinhole alignment complexity. We envision that the proposed DMD-based common-path QPI system will allow for system miniaturization and automation for a broader adaption. National Basic Research Program of China (973 Program) (2015CB352003) National Natural Science Foundation (China) (61335003) National Natural Science Foundation (China) (61427818) National Natural Science Foundation (China) (61378051) Natural Science Foundation of Zhejiang Province (LR16F050001) China. Fundamental Research Funds for the Central Universities (2016FZA5002) National Institutes of Health (U.S.) (1R01HL121386-01A1) National Institutes of Health (U.S.) (9P41EB015871-26A1) Singapore-MIT Alliance for Research and Technology (SMART) Open Foundation of the State Key Laboratory of Modern Optical Instrumentation Hamamatsu Corporation 2019-01-11T20:22:37Z 2019-01-11T20:22:37Z 2017-03 2019-01-04T13:25:47Z Article http://purl.org/eprint/type/JournalArticle 0146-9592 1539-4794 http://hdl.handle.net/1721.1/120010 Zheng, Cheng, Renjie Zhou, Cuifang Kuang, Guangyuan Zhao, Zahid Yaqoob, and Peter T. C. So. “Digital Micromirror Device-Based Common-Path Quantitative Phase Imaging.” Optics Letters 42, no. 7 (March 31, 2017): 1448. © 2017 Optical Society of America. https://orcid.org/0000-0002-4761-6641 https://orcid.org/0000-0003-4698-6488 http://dx.doi.org/10.1364/OL.42.001448 Optics Letters Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf The Optical Society PMC |
| spellingShingle | Zheng, Cheng Zhou, Renjie Kuang, Cuifang Zhao, Guangyuan Yaqoob, Zahid So, Peter T. C. Digital micromirror device-based common-path quantitative phase imaging |
| title | Digital micromirror device-based common-path quantitative phase imaging |
| title_full | Digital micromirror device-based common-path quantitative phase imaging |
| title_fullStr | Digital micromirror device-based common-path quantitative phase imaging |
| title_full_unstemmed | Digital micromirror device-based common-path quantitative phase imaging |
| title_short | Digital micromirror device-based common-path quantitative phase imaging |
| title_sort | digital micromirror device based common path quantitative phase imaging |
| url | http://hdl.handle.net/1721.1/120010 https://orcid.org/0000-0002-4761-6641 https://orcid.org/0000-0003-4698-6488 |
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