Characterization of Lipid Membrane Properties for Tunable Electroporation
Lipid bilayers form nanopores on the application of an electric field. This process of electroporation can be utilized in different applications ranging from targeted drug delivery in cells to nano-gating membrane for engineering applications. However, the ease of electroporation is dependent on the...
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ASME International
2019
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| Online Access: | http://hdl.handle.net/1721.1/120297 https://orcid.org/0000-0002-8272-690X https://orcid.org/0000-0001-7045-1200 |
| _version_ | 1826216570242727936 |
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| author | Cho, Han-Jae Jeremy Maroo, Shalabh Wang, Evelyn |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Cho, Han-Jae Jeremy Maroo, Shalabh Wang, Evelyn |
| author_sort | Cho, Han-Jae Jeremy |
| collection | MIT |
| description | Lipid bilayers form nanopores on the application of an electric field. This process of electroporation can be utilized in different applications ranging from targeted drug delivery in cells to nano-gating membrane for engineering applications. However, the ease of electroporation is dependent on the surface energy of the lipid layers and thus directly related to the packing structure of the lipid molecules. 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid monolayers were deposited on a mica substrate using the Langmuir-Blodgett (LB) technique at different packing densities and analyzed using atomic force microscopy (AFM). The wetting behavior of these monolayers was investigated by contact angle measurement and molecular dynamics simulations. It was found that an equilibrium packing density of liquid-condensed (LC) phase DPPC likely exists and that water molecules can penetrate the monolayer displacing the lipid molecules. The surface tension of the monolayer in air and water was obtained along with its breakthrough force. Topics: Membranes, Electroporation |
| first_indexed | 2024-09-23T16:49:23Z |
| format | Article |
| id | mit-1721.1/120297 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:49:23Z |
| publishDate | 2019 |
| publisher | ASME International |
| record_format | dspace |
| spelling | mit-1721.1/1202972022-09-29T21:46:43Z Characterization of Lipid Membrane Properties for Tunable Electroporation Cho, Han-Jae Jeremy Maroo, Shalabh Wang, Evelyn Massachusetts Institute of Technology. Department of Mechanical Engineering Cho, Han-Jae Jeremy Maroo, Shalabh Wang, Evelyn Lipid bilayers form nanopores on the application of an electric field. This process of electroporation can be utilized in different applications ranging from targeted drug delivery in cells to nano-gating membrane for engineering applications. However, the ease of electroporation is dependent on the surface energy of the lipid layers and thus directly related to the packing structure of the lipid molecules. 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid monolayers were deposited on a mica substrate using the Langmuir-Blodgett (LB) technique at different packing densities and analyzed using atomic force microscopy (AFM). The wetting behavior of these monolayers was investigated by contact angle measurement and molecular dynamics simulations. It was found that an equilibrium packing density of liquid-condensed (LC) phase DPPC likely exists and that water molecules can penetrate the monolayer displacing the lipid molecules. The surface tension of the monolayer in air and water was obtained along with its breakthrough force. Topics: Membranes, Electroporation National Science Foundation (U.S.). Materials Research Science and Engineering Centers (Program) 2019-02-08T18:27:55Z 2019-02-08T18:27:55Z 2012-03 2019-01-09T17:25:31Z Article http://purl.org/eprint/type/ConferencePaper 978-0-7918-5477-8 http://hdl.handle.net/1721.1/120297 Cho, H. Jeremy, Shalabh C. Maroo, and Evelyn N. Wang. “Characterization of Lipid Membrane Properties for Tunable Electroporation.” ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer, 3-6 March 3, 2012, Atlanta, Georgia, ASME, 2012. © 2012 by ASME https://orcid.org/0000-0002-8272-690X https://orcid.org/0000-0001-7045-1200 http://dx.doi.org/10.1115/MNHMT2012-75321 ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf ASME International ASME |
| spellingShingle | Cho, Han-Jae Jeremy Maroo, Shalabh Wang, Evelyn Characterization of Lipid Membrane Properties for Tunable Electroporation |
| title | Characterization of Lipid Membrane Properties for Tunable Electroporation |
| title_full | Characterization of Lipid Membrane Properties for Tunable Electroporation |
| title_fullStr | Characterization of Lipid Membrane Properties for Tunable Electroporation |
| title_full_unstemmed | Characterization of Lipid Membrane Properties for Tunable Electroporation |
| title_short | Characterization of Lipid Membrane Properties for Tunable Electroporation |
| title_sort | characterization of lipid membrane properties for tunable electroporation |
| url | http://hdl.handle.net/1721.1/120297 https://orcid.org/0000-0002-8272-690X https://orcid.org/0000-0001-7045-1200 |
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