Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy
Imaging Fourier-transform spectroscopy (IFTS) is a powerful method for biological hyperspectral analysis based on various imaging modalities, such as fluorescence or Raman. Since the measurements are taken in the Fourier space of the spectrum, it can also take advantage of compressed sensing strateg...
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The Optical Society
2019
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| Online Access: | http://hdl.handle.net/1721.1/120327 https://orcid.org/0000-0001-6880-9509 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0003-4698-6488 |
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| author | Heemskerk, Hans Wadduwage, Dushan Nawoda Singh, Vijay Raj Choi, Heejin Yaqoob, Zahid So, Peter T. C. Matsudaira, Paul T. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Heemskerk, Hans Wadduwage, Dushan Nawoda Singh, Vijay Raj Choi, Heejin Yaqoob, Zahid So, Peter T. C. Matsudaira, Paul T. |
| author_sort | Heemskerk, Hans |
| collection | MIT |
| description | Imaging Fourier-transform spectroscopy (IFTS) is a powerful method for biological hyperspectral analysis based on various imaging modalities, such as fluorescence or Raman. Since the measurements are taken in the Fourier space of the spectrum, it can also take advantage of compressed sensing strategies. IFTS has been readily implemented in high-throughput, high-content microscope systems based on wide-field imaging modalities. However, there are limitations in existing wide-field IFTS designs. Non-common-path approaches are less phase-stable. Alternatively, designs based on the common-path Sagnac interferometer are stable, but incompatible with high-throughput imaging. They require exhaustive sequential scanning over large interferometric path delays, making compressive strategic data acquisition impossible. In this paper, we present a novel phase-stable, near-common-path interferometer enabling high-throughput hyperspectral imaging based on strategic data acquisition. Our results suggest that this approach can improve throughput over those of many other wide-field spectral techniques by more than an order of magnitude without compromising phase stability. |
| first_indexed | 2024-09-23T16:15:57Z |
| format | Article |
| id | mit-1721.1/120327 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:15:57Z |
| publishDate | 2019 |
| publisher | The Optical Society |
| record_format | dspace |
| spelling | mit-1721.1/1203272024-03-20T19:34:18Z Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy Heemskerk, Hans Wadduwage, Dushan Nawoda Singh, Vijay Raj Choi, Heejin Yaqoob, Zahid So, Peter T. C. Matsudaira, Paul T. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Spectroscopy Laboratory Wadduwage, Dushan Nawoda Singh, Vijay Raj Choi, Heejin Yaqoob, Zahid Matsudaira, Paul T So, Peter T. C. Imaging Fourier-transform spectroscopy (IFTS) is a powerful method for biological hyperspectral analysis based on various imaging modalities, such as fluorescence or Raman. Since the measurements are taken in the Fourier space of the spectrum, it can also take advantage of compressed sensing strategies. IFTS has been readily implemented in high-throughput, high-content microscope systems based on wide-field imaging modalities. However, there are limitations in existing wide-field IFTS designs. Non-common-path approaches are less phase-stable. Alternatively, designs based on the common-path Sagnac interferometer are stable, but incompatible with high-throughput imaging. They require exhaustive sequential scanning over large interferometric path delays, making compressive strategic data acquisition impossible. In this paper, we present a novel phase-stable, near-common-path interferometer enabling high-throughput hyperspectral imaging based on strategic data acquisition. Our results suggest that this approach can improve throughput over those of many other wide-field spectral techniques by more than an order of magnitude without compromising phase stability. National Institutes of Health (U.S.) (1R01HL121386-01A1) National Institutes of Health (U.S.) (1U01CA202177-01) National Institutes of Health (U.S.) (4R44EB012415) National Institutes of Health (U.S.) (5R01NS051320) National Institutes of Health (U.S.) (9P41EB015871-26A1) Singapore-MIT Alliance. BioSystems and Micromechanics (BioSyM) Inter-Disciplinary Research Group Hamamatsu Corporation Samsung Advanced Institute of Technology 2019-02-11T18:30:36Z 2019-02-11T18:30:36Z 2017-05 2019-01-04T13:33:39Z Article http://purl.org/eprint/type/JournalArticle 2334-2536 http://hdl.handle.net/1721.1/120327 Wadduwage, Dushan N., Vijay Raj Singh, Heejin Choi, Zahid Yaqoob, Hans Heemskerk, Paul Matsudaira, and Peter T. C. So. “Near-Common-Path Interferometer for Imaging Fourier-Transform Spectroscopy in Wide-Field Microscopy.” Optica 4, no. 5 (May 16, 2017): 546. https://orcid.org/0000-0001-6880-9509 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0003-4698-6488 http://dx.doi.org/10.1364/OPTICA.4.000546 Optica Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf The Optical Society PMC |
| spellingShingle | Heemskerk, Hans Wadduwage, Dushan Nawoda Singh, Vijay Raj Choi, Heejin Yaqoob, Zahid So, Peter T. C. Matsudaira, Paul T. Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy |
| title | Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy |
| title_full | Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy |
| title_fullStr | Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy |
| title_full_unstemmed | Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy |
| title_short | Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy |
| title_sort | near common path interferometer for imaging fourier transform spectroscopy in wide field microscopy |
| url | http://hdl.handle.net/1721.1/120327 https://orcid.org/0000-0001-6880-9509 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0003-4698-6488 |
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