High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap
We report a method to achieve high speed and high resolution live cell Raman images using small spherical gold nanoparticles with highly narrow intra-nanogap structures responding to NIR excitation (785 nm) and high-speed confocal Raman microscopy. The three different Raman-active molecules placed i...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Published: |
American Chemical Society (ACS)
2019
|
| Online Access: | http://hdl.handle.net/1721.1/120337 https://orcid.org/0000-0003-2012-9023 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1811069013293268992 |
|---|---|
| author | Kang, Jeon Woong So, Peter T. C. Dasari, Ramachandra Rao Lim, Dong Kwon |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Kang, Jeon Woong So, Peter T. C. Dasari, Ramachandra Rao Lim, Dong Kwon |
| author_sort | Kang, Jeon Woong |
| collection | MIT |
| description | We report a method to achieve high speed and high resolution live cell Raman images using small spherical gold nanoparticles with highly narrow intra-nanogap structures responding to NIR excitation (785 nm) and high-speed confocal Raman microscopy. The three different Raman-active molecules placed in the narrow intra-nanogap showed a strong and uniform Raman intensity in solution even under transient exposure time (10 ms) and low input power of incident laser (200 μW), which lead to obtain high-resolution single cell image within 30 s without inducing significant cell damage. The high resolution Raman image showed the distributions of gold nanoparticles for their targeted sites such as cytoplasm, mitochondria, or nucleus. The high speed Raman-based live cell imaging allowed us to monitor rapidly changing cell morphologies during cell death induced by the addition of highly toxic KCN solution to cells. These results strongly suggest that the use of SERS-active nanoparticle can greatly improve the current temporal resolution and image quality of Raman-based cell images enough to obtain the detailed cell dynamics and/or the responses of cells to potential drug molecules. |
| first_indexed | 2024-09-23T08:04:19Z |
| format | Article |
| id | mit-1721.1/120337 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T08:04:19Z |
| publishDate | 2019 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1203372022-09-23T10:43:30Z High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap Kang, Jeon Woong So, Peter T. C. Dasari, Ramachandra Rao Lim, Dong Kwon Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Spectroscopy Laboratory Koch Institute for Integrative Cancer Research at MIT Kang, Jeon Woong So, Peter T. C. Dasari, Ramachandra Rao Lim, Dong Kwon We report a method to achieve high speed and high resolution live cell Raman images using small spherical gold nanoparticles with highly narrow intra-nanogap structures responding to NIR excitation (785 nm) and high-speed confocal Raman microscopy. The three different Raman-active molecules placed in the narrow intra-nanogap showed a strong and uniform Raman intensity in solution even under transient exposure time (10 ms) and low input power of incident laser (200 μW), which lead to obtain high-resolution single cell image within 30 s without inducing significant cell damage. The high resolution Raman image showed the distributions of gold nanoparticles for their targeted sites such as cytoplasm, mitochondria, or nucleus. The high speed Raman-based live cell imaging allowed us to monitor rapidly changing cell morphologies during cell death induced by the addition of highly toxic KCN solution to cells. These results strongly suggest that the use of SERS-active nanoparticle can greatly improve the current temporal resolution and image quality of Raman-based cell images enough to obtain the detailed cell dynamics and/or the responses of cells to potential drug molecules. National Research Foundation of Korea (NRF-2013R1A1A1061387) Jeon-Dae Memorial Fund National Institutes of Health (U.S.) (9P41EB015871-27) MIT Skoltech Initiative National Institutes of Health (U.S.) (5R01EY017656-02) National Institutes of Health (U.S.) (5R01NS051320) National Institutes of Health (U.S.) (4R44EB012415-02) National Science Foundation (U.S.). Chemical, Bioengineering, Enviromental, and Transport Systems (CBET-0939511) Singapore-MIT Alliance Hamamatsu Corporation Koch Institute for Integrative Cancer Research. Bridge Project Initiative 2019-02-12T14:48:37Z 2019-02-12T14:48:37Z 2015-02 2019-01-03T18:52:47Z Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/120337 Kang, Jeon Woong, Peter T. C. So, Ramachandra R. Dasari, and Dong-Kwon Lim. “High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap.” Nano Letters 15, no. 3 (February 11, 2015): 1766–1772. https://orcid.org/0000-0003-2012-9023 https://orcid.org/0000-0003-4698-6488 http://dx.doi.org/10.1021/NL504444W Nano Letters Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Kang, Jeon Woong So, Peter T. C. Dasari, Ramachandra Rao Lim, Dong Kwon High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap |
| title | High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap |
| title_full | High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap |
| title_fullStr | High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap |
| title_full_unstemmed | High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap |
| title_short | High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap |
| title_sort | high resolution live cell raman imaging using subcellular organelle targeting sers sensitive gold nanoparticles with highly narrow intra nanogap |
| url | http://hdl.handle.net/1721.1/120337 https://orcid.org/0000-0003-2012-9023 https://orcid.org/0000-0003-4698-6488 |
| work_keys_str_mv | AT kangjeonwoong highresolutionlivecellramanimagingusingsubcellularorganelletargetingserssensitivegoldnanoparticleswithhighlynarrowintrananogap AT sopetertc highresolutionlivecellramanimagingusingsubcellularorganelletargetingserssensitivegoldnanoparticleswithhighlynarrowintrananogap AT dasariramachandrarao highresolutionlivecellramanimagingusingsubcellularorganelletargetingserssensitivegoldnanoparticleswithhighlynarrowintrananogap AT limdongkwon highresolutionlivecellramanimagingusingsubcellularorganelletargetingserssensitivegoldnanoparticleswithhighlynarrowintrananogap |