Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima
Gold nanoparticles are quite popular as contrast agents for optical microscopy. Their strong linear and nonlinear interaction with light, coupled with their biocompatibility and resistance to photobleaching make them suitable contrasts agents for bioimaging applications. Gold nanorods have been used...
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SPIE-Intl Soc Optical Eng
2019
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| Online Access: | http://hdl.handle.net/1721.1/120344 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826189254573686784 |
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| author | Balla, Naveen Sheppard, Colin So, Peter T. C. |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Balla, Naveen Sheppard, Colin So, Peter T. C. |
| author_sort | Balla, Naveen |
| collection | MIT |
| description | Gold nanoparticles are quite popular as contrast agents for optical microscopy. Their strong linear and nonlinear interaction with light, coupled with their biocompatibility and resistance to photobleaching make them suitable contrasts agents for bioimaging applications. Gold nanorods have been used for in vivo two photon microscopy in small animals [PNAS 102, 15752 (2005)]. Conventional two photon microscopy with gold nanorods involves exciting these particles with femtosecond laser at wavelengths close to their longitudinal plasmon resonance (LPR). Most of the reported works used Ti:Sapphire laser with excitation wavelengths ranging from 780 nm to 850 nm. The rational was to maximize absorption of excitation wavelengths, a fraction of which gives rise to two photon luminescence. This however causes intense heating of the nanorods and unless the excitation powers are kept low, gold nanorods tend to melt [Phys Rev Lett 95, 267405 (2005)]. Another less explored way of getting multiphoton emission from gold nanorods is to excite them at long wavelengths far away from their LPR wavelength [Jour Amer Chem Soc 131, 14186 (2009)]. We are interested in femtosecond lasers operating around 1200 nm wavelengths because of their lower scattering and absorption by tissue and water. Here we compare multiphoton photon luminescence properties of gold nanorods when excited at wavelengths around 800 nm and 1200 nm. Excitation with wavelengths around 1200 nm has certain advantages like lower heating of the particles and hence prolonged durations of imaging. Other advantage is the ability to collect emission in the near infrared regions (NIR) up to 800 nm which is not possible when using excitation wavelengths around 800 nm. These features are good for deep tissue imaging. One disadvantage of this approach is lower luminescence intensity. |
| first_indexed | 2024-09-23T08:12:14Z |
| format | Article |
| id | mit-1721.1/120344 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T08:12:14Z |
| publishDate | 2019 |
| publisher | SPIE-Intl Soc Optical Eng |
| record_format | dspace |
| spelling | mit-1721.1/1203442022-09-23T11:36:10Z Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima Balla, Naveen Sheppard, Colin So, Peter T. C. Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Balla, Naveen Sheppard, Colin So, Peter T. C. Gold nanoparticles are quite popular as contrast agents for optical microscopy. Their strong linear and nonlinear interaction with light, coupled with their biocompatibility and resistance to photobleaching make them suitable contrasts agents for bioimaging applications. Gold nanorods have been used for in vivo two photon microscopy in small animals [PNAS 102, 15752 (2005)]. Conventional two photon microscopy with gold nanorods involves exciting these particles with femtosecond laser at wavelengths close to their longitudinal plasmon resonance (LPR). Most of the reported works used Ti:Sapphire laser with excitation wavelengths ranging from 780 nm to 850 nm. The rational was to maximize absorption of excitation wavelengths, a fraction of which gives rise to two photon luminescence. This however causes intense heating of the nanorods and unless the excitation powers are kept low, gold nanorods tend to melt [Phys Rev Lett 95, 267405 (2005)]. Another less explored way of getting multiphoton emission from gold nanorods is to excite them at long wavelengths far away from their LPR wavelength [Jour Amer Chem Soc 131, 14186 (2009)]. We are interested in femtosecond lasers operating around 1200 nm wavelengths because of their lower scattering and absorption by tissue and water. Here we compare multiphoton photon luminescence properties of gold nanorods when excited at wavelengths around 800 nm and 1200 nm. Excitation with wavelengths around 1200 nm has certain advantages like lower heating of the particles and hence prolonged durations of imaging. Other advantage is the ability to collect emission in the near infrared regions (NIR) up to 800 nm which is not possible when using excitation wavelengths around 800 nm. These features are good for deep tissue imaging. One disadvantage of this approach is lower luminescence intensity. Singapore-MIT Alliance 2019-02-12T17:06:48Z 2019-02-12T17:06:48Z 2011-02 2019-01-03T17:33:18Z Article http://purl.org/eprint/type/ConferencePaper http://hdl.handle.net/1721.1/120344 Balla, Naveen K., Colin J. R. Sheppard, and Peter T. C. So. “Multiphoton Luminescence of Gold Nanorods Upon Excitation with Wavelengths Away from Their Absorption Maxima.” Edited by Samuel Achilefu and Ramesh Raghavachari. Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III (February 10, 2011). https://orcid.org/0000-0003-4698-6488 http://dx.doi.org/10.1117/12.876014 Biomedical applications Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE-Intl Soc Optical Eng SPIE |
| spellingShingle | Balla, Naveen Sheppard, Colin So, Peter T. C. Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| title | Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| title_full | Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| title_fullStr | Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| title_full_unstemmed | Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| title_short | Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| title_sort | multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima |
| url | http://hdl.handle.net/1721.1/120344 https://orcid.org/0000-0003-4698-6488 |
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