Folding artificial mucosa with cell-laden hydrogels guided by mechanics models
The surfaces of many hollow or tubular tissues/organs in our respiratory, gastrointestinal, and urogenital tracts are covered by mucosa with folded patterns. The patterns are induced by mechanical instability of the mucosa under compression due to constrained growth. Recapitulating this folding proc...
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| Format: | Article |
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National Academy of Sciences (U.S.)
2019
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| Online Access: | http://hdl.handle.net/1721.1/120544 https://orcid.org/0000-0002-9292-5267 https://orcid.org/0000-0001-7922-0249 https://orcid.org/0000-0002-1801-5548 https://orcid.org/0000-0001-5387-6186 |
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| author | Meng, Hu Leong, Kam W. Chan, Hon Fai Zhao, Ruike Parada Hernandez, German Alberto Griffith, Linda G Zhao, Xuanhe |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Meng, Hu Leong, Kam W. Chan, Hon Fai Zhao, Ruike Parada Hernandez, German Alberto Griffith, Linda G Zhao, Xuanhe |
| author_sort | Meng, Hu |
| collection | MIT |
| description | The surfaces of many hollow or tubular tissues/organs in our respiratory, gastrointestinal, and urogenital tracts are covered by mucosa with folded patterns. The patterns are induced by mechanical instability of the mucosa under compression due to constrained growth. Recapitulating this folding process in vitro will facilitate the understanding and engineering of mucosa in various tissues/organs. However, scant attention has been paid to address the challenge of reproducing mucosal folding. Here we mimic the mucosal folding process using a cell-laden hydrogel film attached to a prestretched tough-hydrogel substrate. The cell-laden hydrogel constitutes a human epithelial cell lining on stromal component to recapitulate the physiological feature of a mucosa. Relaxation of the prestretched tough-hydrogel substrate applies compressive strains on the cell-laden hydrogel film, which undergoes mechanical instability and evolves into morphological patterns. We predict the conditions for mucosal folding as well as the morphology of and strain in the folded artificial mucosa using a combination of theory and simulation. The work not only provides a simple method to fold artificial mucosa but also demonstrates a paradigm in tissue engineering via harnessing mechanical instabilities guided by quantitative mechanics models. Keywords: mucosa; hydrogel; mechanical instabiity; tissue engineering; biomechanics |
| first_indexed | 2024-09-23T14:05:58Z |
| format | Article |
| id | mit-1721.1/120544 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:05:58Z |
| publishDate | 2019 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1205442022-09-28T18:26:30Z Folding artificial mucosa with cell-laden hydrogels guided by mechanics models Meng, Hu Leong, Kam W. Chan, Hon Fai Zhao, Ruike Parada Hernandez, German Alberto Griffith, Linda G Zhao, Xuanhe Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Chan, Hon Fai Zhao, Ruike Parada Hernandez, German Alberto Griffith, Linda G Zhao, Xuanhe The surfaces of many hollow or tubular tissues/organs in our respiratory, gastrointestinal, and urogenital tracts are covered by mucosa with folded patterns. The patterns are induced by mechanical instability of the mucosa under compression due to constrained growth. Recapitulating this folding process in vitro will facilitate the understanding and engineering of mucosa in various tissues/organs. However, scant attention has been paid to address the challenge of reproducing mucosal folding. Here we mimic the mucosal folding process using a cell-laden hydrogel film attached to a prestretched tough-hydrogel substrate. The cell-laden hydrogel constitutes a human epithelial cell lining on stromal component to recapitulate the physiological feature of a mucosa. Relaxation of the prestretched tough-hydrogel substrate applies compressive strains on the cell-laden hydrogel film, which undergoes mechanical instability and evolves into morphological patterns. We predict the conditions for mucosal folding as well as the morphology of and strain in the folded artificial mucosa using a combination of theory and simulation. The work not only provides a simple method to fold artificial mucosa but also demonstrates a paradigm in tissue engineering via harnessing mechanical instabilities guided by quantitative mechanics models. Keywords: mucosa; hydrogel; mechanical instabiity; tissue engineering; biomechanics National Science Foundation (U.S.) (Award CMMI-1661627) United States. Office of Naval Research (Award N00014-17-1-2920) 2019-02-22T18:35:27Z 2019-02-22T18:35:27Z 2018-07 2018-02 2019-02-08T18:16:05Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/120544 Chan, Hon Fai et al. “Folding Artificial Mucosa with Cell-Laden Hydrogels Guided by Mechanics Models.” Proceedings of the National Academy of Sciences 115, 29 (July 2018): 7503–7508 © 2018 National Academy of Sciences https://orcid.org/0000-0002-9292-5267 https://orcid.org/0000-0001-7922-0249 https://orcid.org/0000-0002-1801-5548 https://orcid.org/0000-0001-5387-6186 http://dx.doi.org/10.1073/pnas.1802361115 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Meng, Hu Leong, Kam W. Chan, Hon Fai Zhao, Ruike Parada Hernandez, German Alberto Griffith, Linda G Zhao, Xuanhe Folding artificial mucosa with cell-laden hydrogels guided by mechanics models |
| title | Folding artificial mucosa with cell-laden hydrogels guided by mechanics models |
| title_full | Folding artificial mucosa with cell-laden hydrogels guided by mechanics models |
| title_fullStr | Folding artificial mucosa with cell-laden hydrogels guided by mechanics models |
| title_full_unstemmed | Folding artificial mucosa with cell-laden hydrogels guided by mechanics models |
| title_short | Folding artificial mucosa with cell-laden hydrogels guided by mechanics models |
| title_sort | folding artificial mucosa with cell laden hydrogels guided by mechanics models |
| url | http://hdl.handle.net/1721.1/120544 https://orcid.org/0000-0002-9292-5267 https://orcid.org/0000-0001-7922-0249 https://orcid.org/0000-0002-1801-5548 https://orcid.org/0000-0001-5387-6186 |
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