Topology effects on protein–polymer block copolymer self-assembly
Bioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show that topology, i.e. the BA, BA2, ABA and ABC chain structure of the block copolymers, where B represents the protein and A and C represent polymers, has a significant effect on ordering transitions an...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Royal Society of Chemistry, The
2019
|
| Online Access: | http://hdl.handle.net/1721.1/120749 https://orcid.org/0000-0002-7272-7140 |
| _version_ | 1811069329652842496 |
|---|---|
| author | Suguri, Takuya Olsen, Bradley D |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Suguri, Takuya Olsen, Bradley D |
| author_sort | Suguri, Takuya |
| collection | MIT |
| description | Bioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show
that topology, i.e. the BA, BA2, ABA and ABC chain structure of the block copolymers, where B represents
the protein and A and C represent polymers, has a significant effect on ordering transitions and the type
and size of nanostructures formed during microphase separation. ABA and ABC type block copolymers
were synthesized by using two site-specific bioconjugation reactions: the thiol–ene reaction with a
cysteine on mCherry and maleimide functionalized polymers, and the sortase A ligation reaction with an
LPETG sequence at the C-terminus on mCherry and a triglycine functionalized polymer. The phase behaviors of mCherry–poly(N-isopropylacrylamide) (PNIPAM) and mCherry–(PNIPAM)2 show that the shapes
of the phase diagrams are similar overall, but mCherry–(PNIPAM)2, i.e. BA2 type, yields a narrower domain
spacing than mCherry–PNIPAM, i.e. BA type. PNIPAM–mCherry–PNIPAM (ABA type) shows only lamellar
phases in the range of conditions under which ordered structures appear. PDMAPS–mCherry–PNIPAM
(ABC type) shows an ordered structure across the widest range of conditions in the four bioconjugates
and also the widest range of different nanodomain structures. The phase behavior of the ABC type implies
that the repulsive interaction between two water-soluble coil polymers can be a key factor in enhancing
the self-assembly of globular protein–polymer block copolymers. |
| first_indexed | 2024-09-23T08:09:23Z |
| format | Article |
| id | mit-1721.1/120749 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:09:23Z |
| publishDate | 2019 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/1207492022-09-23T11:17:58Z Topology effects on protein–polymer block copolymer self-assembly Suguri, Takuya Olsen, Bradley D Massachusetts Institute of Technology. Department of Chemical Engineering Suguri, Takuya Olsen, Bradley D Bioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show that topology, i.e. the BA, BA2, ABA and ABC chain structure of the block copolymers, where B represents the protein and A and C represent polymers, has a significant effect on ordering transitions and the type and size of nanostructures formed during microphase separation. ABA and ABC type block copolymers were synthesized by using two site-specific bioconjugation reactions: the thiol–ene reaction with a cysteine on mCherry and maleimide functionalized polymers, and the sortase A ligation reaction with an LPETG sequence at the C-terminus on mCherry and a triglycine functionalized polymer. The phase behaviors of mCherry–poly(N-isopropylacrylamide) (PNIPAM) and mCherry–(PNIPAM)2 show that the shapes of the phase diagrams are similar overall, but mCherry–(PNIPAM)2, i.e. BA2 type, yields a narrower domain spacing than mCherry–PNIPAM, i.e. BA type. PNIPAM–mCherry–PNIPAM (ABA type) shows only lamellar phases in the range of conditions under which ordered structures appear. PDMAPS–mCherry–PNIPAM (ABC type) shows an ordered structure across the widest range of conditions in the four bioconjugates and also the widest range of different nanodomain structures. The phase behavior of the ABC type implies that the repulsive interaction between two water-soluble coil polymers can be a key factor in enhancing the self-assembly of globular protein–polymer block copolymers. Department of Energy Office of Basic Energy Sciences (Award DE-SC0007106) 2019-03-05T20:34:00Z 2019-03-05T20:34:00Z 2019-03 2018-08 Article http://purl.org/eprint/type/JournalArticle 1759-9954 1759-9962 http://hdl.handle.net/1721.1/120749 Suguri, Takuya, and Bradley D. Olsen. “Topology Effects on Protein–polymer Block Copolymer Self-Assembly.” Polymer Chemistry (2019). doi:10.1039/c8py01228h. https://orcid.org/0000-0002-7272-7140 en_US https://doi.org/10.1039/C8PY01228H Polymer Chemistry Creative Commons Attribution Noncommercial 3.0 unported license https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry, The Royal Society of Chemistry (RSC) |
| spellingShingle | Suguri, Takuya Olsen, Bradley D Topology effects on protein–polymer block copolymer self-assembly |
| title | Topology effects on protein–polymer block copolymer self-assembly |
| title_full | Topology effects on protein–polymer block copolymer self-assembly |
| title_fullStr | Topology effects on protein–polymer block copolymer self-assembly |
| title_full_unstemmed | Topology effects on protein–polymer block copolymer self-assembly |
| title_short | Topology effects on protein–polymer block copolymer self-assembly |
| title_sort | topology effects on protein polymer block copolymer self assembly |
| url | http://hdl.handle.net/1721.1/120749 https://orcid.org/0000-0002-7272-7140 |
| work_keys_str_mv | AT suguritakuya topologyeffectsonproteinpolymerblockcopolymerselfassembly AT olsenbradleyd topologyeffectsonproteinpolymerblockcopolymerselfassembly |