Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures
Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient...
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American Association for the Advancement of Science (AAAS)
2019
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| Rochtain ar líne: | http://hdl.handle.net/1721.1/120835 https://orcid.org/0000-0001-7014-3830 |
| _version_ | 1826207128461770752 |
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| author | Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Reyes, Miguel Blainey, Paul C |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Reyes, Miguel Blainey, Paul C |
| author_sort | Vickers, Dwayne |
| collection | MIT |
| description | Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system’s technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings. |
| first_indexed | 2024-09-23T13:44:53Z |
| format | Article |
| id | mit-1721.1/120835 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:44:53Z |
| publishDate | 2019 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1208352022-10-01T16:53:33Z Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Reyes, Miguel Blainey, Paul C Massachusetts Institute of Technology. Department of Biological Engineering Reyes, Miguel Blainey, Paul C Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system’s technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings. National Institute of Allergy and Infectious Diseases (U.S.) (Grant U24 AI118668) 2019-03-08T17:18:17Z 2019-03-08T17:18:17Z 2019-01 2018-07 2019-02-15T14:35:46Z Article http://purl.org/eprint/type/JournalArticle 2375-2548 http://hdl.handle.net/1721.1/120835 Reyes, Miguel et al. “Multiplexed Enrichment and Genomic Profiling of Peripheral Blood Cells Reveal Subset-Specific Immune Signatures.” Science Advances 5, 1 (January 2019): eaau9223 © 2019 The Authors https://orcid.org/0000-0001-7014-3830 http://dx.doi.org/10.1126/sciadv.aau9223 Science Advances Creative Commons Attribution NonCommercial License 4.0 https://creativecommons.org/licenses/by-nc/4.0/ application/pdf American Association for the Advancement of Science (AAAS) Science Advances |
| spellingShingle | Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Reyes, Miguel Blainey, Paul C Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
| title | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
| title_full | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
| title_fullStr | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
| title_full_unstemmed | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
| title_short | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
| title_sort | multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset specific immune signatures |
| url | http://hdl.handle.net/1721.1/120835 https://orcid.org/0000-0001-7014-3830 |
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