tRNA epitranscriptomics and biased codon are linked to proteome expression in
Among components of the translational machinery, ribonucleoside modifications on tRNAs are emerging as critical regulators of cell physiology and stress response. Here, we demonstrate highly coordinated behavior of the repertoire of tRNA modifications of Plasmodium falciparum throughout the intra-er...
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| Format: | Article |
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Nature Publishing Group
2019
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| Online Access: | http://hdl.handle.net/1721.1/121104 https://orcid.org/0000-0001-9920-2080 https://orcid.org/0000-0001-7380-4075 https://orcid.org/0000-0003-0011-3067 |
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| author | Ng, Chee Sheng Sinha, Ameya Aniweh, Yaw Nah, Qianhui Babu, Indrakanti Ramesh Gu, Chen Chionh, Yok Hian Dedon, Peter C Preiser, Peter R |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ng, Chee Sheng Sinha, Ameya Aniweh, Yaw Nah, Qianhui Babu, Indrakanti Ramesh Gu, Chen Chionh, Yok Hian Dedon, Peter C Preiser, Peter R |
| author_sort | Ng, Chee Sheng |
| collection | MIT |
| description | Among components of the translational machinery, ribonucleoside modifications on tRNAs are emerging as critical regulators of cell physiology and stress response. Here, we demonstrate highly coordinated behavior of the repertoire of tRNA modifications of Plasmodium falciparum throughout the intra-erythrocytic developmental cycle (IDC). We observed both a synchronized increase in 22 of 28 modifications from ring to trophozoite stage, consistent with tRNA maturation during translational up-regulation, and asynchronous changes in six modifications. Quantitative analysis of ~2,100 proteins across the IDC revealed that up- and down-regulated proteins in late but not early stages have a marked codon bias that directly correlates with parallel changes in tRNA modifications and enhanced translational efficiency. We thus propose a model in which tRNA modifications modulate the abundance of stage-specific proteins by enhancing translation efficiency of codon-biased transcripts for critical genes. These findings reveal novel epitranscriptomic and translational control mechanisms in the development and pathogenesis of Plasmodium parasites. |
| first_indexed | 2024-09-23T09:37:17Z |
| format | Article |
| id | mit-1721.1/121104 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T09:37:17Z |
| publishDate | 2019 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1211042022-09-26T12:43:18Z tRNA epitranscriptomics and biased codon are linked to proteome expression in Ng, Chee Sheng Sinha, Ameya Aniweh, Yaw Nah, Qianhui Babu, Indrakanti Ramesh Gu, Chen Chionh, Yok Hian Dedon, Peter C Preiser, Peter R Massachusetts Institute of Technology. Department of Biological Engineering Ng, Chee Sheng Sinha, Ameya Gu, Chen Babu, Indrakanti Ramesh Chionh, Yok Hian Dedon, Peter C Among components of the translational machinery, ribonucleoside modifications on tRNAs are emerging as critical regulators of cell physiology and stress response. Here, we demonstrate highly coordinated behavior of the repertoire of tRNA modifications of Plasmodium falciparum throughout the intra-erythrocytic developmental cycle (IDC). We observed both a synchronized increase in 22 of 28 modifications from ring to trophozoite stage, consistent with tRNA maturation during translational up-regulation, and asynchronous changes in six modifications. Quantitative analysis of ~2,100 proteins across the IDC revealed that up- and down-regulated proteins in late but not early stages have a marked codon bias that directly correlates with parallel changes in tRNA modifications and enhanced translational efficiency. We thus propose a model in which tRNA modifications modulate the abundance of stage-specific proteins by enhancing translation efficiency of codon-biased transcripts for critical genes. These findings reveal novel epitranscriptomic and translational control mechanisms in the development and pathogenesis of Plasmodium parasites. Singapore. National Research Foundation Singapore-MIT Alliance (Graduate Fellowship) 2019-03-26T15:17:51Z 2019-03-26T15:17:51Z 2018-10 2018-08 2019-02-28T14:29:54Z Article http://purl.org/eprint/type/JournalArticle 1744-4292 1744-4292 1744-4292 http://hdl.handle.net/1721.1/121104 Ng, Chee Sheng, Ameya Sinha, Yaw Aniweh, Qianhui Nah, Indrakanti Ramesh Babu, Chen Gu, Yok Hian Chionh, Peter C Dedon, and Peter R Preiser. “tRNA Epitranscriptomics and Biased Codon Are Linked to Proteome Expression in Plasmodium Falciparum.” Molecular Systems Biology 14, no. 10 (October 2018): e8009. https://orcid.org/0000-0001-9920-2080 https://orcid.org/0000-0001-7380-4075 https://orcid.org/0000-0003-0011-3067 http://dx.doi.org/10.15252/msb.20178009 Molecular Systems Biology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Molecular Systems Biology |
| spellingShingle | Ng, Chee Sheng Sinha, Ameya Aniweh, Yaw Nah, Qianhui Babu, Indrakanti Ramesh Gu, Chen Chionh, Yok Hian Dedon, Peter C Preiser, Peter R tRNA epitranscriptomics and biased codon are linked to proteome expression in |
| title | tRNA epitranscriptomics and biased codon are linked to proteome expression in |
| title_full | tRNA epitranscriptomics and biased codon are linked to proteome expression in |
| title_fullStr | tRNA epitranscriptomics and biased codon are linked to proteome expression in |
| title_full_unstemmed | tRNA epitranscriptomics and biased codon are linked to proteome expression in |
| title_short | tRNA epitranscriptomics and biased codon are linked to proteome expression in |
| title_sort | trna epitranscriptomics and biased codon are linked to proteome expression in |
| url | http://hdl.handle.net/1721.1/121104 https://orcid.org/0000-0001-9920-2080 https://orcid.org/0000-0001-7380-4075 https://orcid.org/0000-0003-0011-3067 |
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