Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
Background In the ultrahigh molecular weight polyethylene (UHMWPE) prosthetic environment, fibroblasts affected by wear particles have the capacity of osteogenesis to reduce osteolysis. We aimed to assess the effects of macrophages on the osteogenic capability of fibroblasts treated with UHMWPE wea...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
BioMed Central Ltd.
2019
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| Online Access: | http://hdl.handle.net/1721.1/121127 |
| _version_ | 1811080182233038848 |
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| author | Lei, Pengfei Dai, Zixun Zhang, Yu S Niu, Wanting Li, Kun Wang, Long Hu, Yihe Xie, Jie Zhang, Yu Shrike Liu, Huan |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Lei, Pengfei Dai, Zixun Zhang, Yu S Niu, Wanting Li, Kun Wang, Long Hu, Yihe Xie, Jie Zhang, Yu Shrike Liu, Huan |
| author_sort | Lei, Pengfei |
| collection | MIT |
| description | Background
In the ultrahigh molecular weight polyethylene (UHMWPE) prosthetic environment, fibroblasts affected by wear particles have the capacity of osteogenesis to reduce osteolysis. We aimed to assess the effects of macrophages on the osteogenic capability of fibroblasts treated with UHMWPE wear particles.
Methods
The effect of different concentrations of UHMWPE (0, 0.01, 0.1, and 1 mg/ml, respectively) on macrophage proliferation were validated by MTT assay to determine the optimum one. The fibroblasts viability was further determined in the co-culture system of UHMWPE particles and macrophage supernatants. The experiment was designed as seven groups: (A) fibroblasts only; (B) fibroblasts + 1 mg/ml UHMWPE particles; and (C1–C5) fibroblasts + 1/16, 1/8, 1/4, 1/2, and 1/1 supernatants of macrophage cultures stimulated by 1 mg/ml UHMWPE particles vs. fibroblast complete media, respectively. Alizarin red staining was used to detect calcium accumulation. The expression levels of osteogenic proteins were detected by Western blot and ELISA, including alkaline phosphatase (ALP) and osteocalcin (OCN).
Results
The concentration of 0.1 mg/ml was considered as the optimum concentration for macrophage proliferation due to the survival rate and was highest among the four concentrations. Fibroblast viability was better in the group of fibroblasts + 1/16 ratio of macrophage supernatants stimulated by 1 mg/ml of UHMWPE particles than the other groups (1:8, 1:4, 1:2, 1:1). ALP and OCN expressions were significantly decreased in the group of fibroblasts + 1/4, 1/2, and 1/1 supernatants stimulated by 1 mg/ml of UHMWPE particles compared with other groups (1/8, 1/16) and the group of fibroblasts + 1 mg/ml UHMWPE (p < 0.5).
Conclusions
Macrophages are potentially involved in the periprosthetic osteolysis by reducing the osteogenic capability of fibroblasts treated with wear particles generated from UHMWPE materials in total hip arthroplasty. |
| first_indexed | 2024-09-23T11:27:10Z |
| format | Article |
| id | mit-1721.1/121127 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:27:10Z |
| publishDate | 2019 |
| publisher | BioMed Central Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/1211272022-09-27T19:38:25Z Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis Lei, Pengfei Dai, Zixun Zhang, Yu S Niu, Wanting Li, Kun Wang, Long Hu, Yihe Xie, Jie Zhang, Yu Shrike Liu, Huan Harvard University--MIT Division of Health Sciences and Technology Liu, Huan Background In the ultrahigh molecular weight polyethylene (UHMWPE) prosthetic environment, fibroblasts affected by wear particles have the capacity of osteogenesis to reduce osteolysis. We aimed to assess the effects of macrophages on the osteogenic capability of fibroblasts treated with UHMWPE wear particles. Methods The effect of different concentrations of UHMWPE (0, 0.01, 0.1, and 1 mg/ml, respectively) on macrophage proliferation were validated by MTT assay to determine the optimum one. The fibroblasts viability was further determined in the co-culture system of UHMWPE particles and macrophage supernatants. The experiment was designed as seven groups: (A) fibroblasts only; (B) fibroblasts + 1 mg/ml UHMWPE particles; and (C1–C5) fibroblasts + 1/16, 1/8, 1/4, 1/2, and 1/1 supernatants of macrophage cultures stimulated by 1 mg/ml UHMWPE particles vs. fibroblast complete media, respectively. Alizarin red staining was used to detect calcium accumulation. The expression levels of osteogenic proteins were detected by Western blot and ELISA, including alkaline phosphatase (ALP) and osteocalcin (OCN). Results The concentration of 0.1 mg/ml was considered as the optimum concentration for macrophage proliferation due to the survival rate and was highest among the four concentrations. Fibroblast viability was better in the group of fibroblasts + 1/16 ratio of macrophage supernatants stimulated by 1 mg/ml of UHMWPE particles than the other groups (1:8, 1:4, 1:2, 1:1). ALP and OCN expressions were significantly decreased in the group of fibroblasts + 1/4, 1/2, and 1/1 supernatants stimulated by 1 mg/ml of UHMWPE particles compared with other groups (1/8, 1/16) and the group of fibroblasts + 1 mg/ml UHMWPE (p < 0.5). Conclusions Macrophages are potentially involved in the periprosthetic osteolysis by reducing the osteogenic capability of fibroblasts treated with wear particles generated from UHMWPE materials in total hip arthroplasty. China Sholarship Council (Grant 201506370173) China. National Natural Science Foundation (Grant 31200739) China. National Natural Science Foundation (Grant 31470948) 2019-03-29T20:54:19Z 2019-03-29T20:54:19Z 2019-03 2018-08 2019-03-24T04:19:06Z Article http://purl.org/eprint/type/JournalArticle 1749-799X http://hdl.handle.net/1721.1/121127 Lei, Pengfei et al. "Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis." Journal of Orthopaedic Surgery and Research 14, 80 (March 2019) https://doi.org/10.1186/s13018-019-1119-8 © The Author(s) en https://doi.org/10.1186/s13018-019-1119-8 Journal of Orthopaedic Surgery and Research Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ The Author(s). application/pdf BioMed Central Ltd. BioMed Central |
| spellingShingle | Lei, Pengfei Dai, Zixun Zhang, Yu S Niu, Wanting Li, Kun Wang, Long Hu, Yihe Xie, Jie Zhang, Yu Shrike Liu, Huan Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| title | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| title_full | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| title_fullStr | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| title_full_unstemmed | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| title_short | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| title_sort | macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene uhmwpe wear particle induced osteolysis |
| url | http://hdl.handle.net/1721.1/121127 |
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