Deep-tissue optical imaging of near cellular-sized features
Detection of biological features at the cellular level with sufcient sensitivity in complex tissue remains a major challenge. To appreciate this challenge, this would require fnding tens to hundreds of cells (a 0.1 mm tumor has ~125 cells), out of ~37 trillion cells in the human body. Near-infrared...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2019
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| Online Access: | http://hdl.handle.net/1721.1/121128 https://orcid.org/0000-0002-4343-4007 https://orcid.org/0000-0002-7530-4725 https://orcid.org/0000-0001-5646-1007 https://orcid.org/0000-0002-4171-3547 https://orcid.org/0000-0001-9353-7453 |
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| author | Dang, Xiangnan Bardhan, Neelkanth Manoj Qi, Jifa Gu, Li Eze, Ngozi A Lin, Ching-Wei Kataria, Swati Hammond, Paula T Belcher, Angela M |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Dang, Xiangnan Bardhan, Neelkanth Manoj Qi, Jifa Gu, Li Eze, Ngozi A Lin, Ching-Wei Kataria, Swati Hammond, Paula T Belcher, Angela M |
| author_sort | Dang, Xiangnan |
| collection | MIT |
| description | Detection of biological features at the cellular level with sufcient sensitivity in complex tissue remains a major challenge. To appreciate this challenge, this would require fnding tens to hundreds of cells (a 0.1 mm tumor has ~125 cells), out of ~37 trillion cells in the human body. Near-infrared optical imaging holds promise for high-resolution, deep-tissue imaging, but is limited by autofuorescence and scattering. To date, the maximum reported depth using second-window near-infrared (NIR-II: 1000–1700 nm) fuorophores is 3.2 cm through tissue. Here, we design an NIR-II imaging system, “Detection of Optically Luminescent Probes using Hyperspectral and difuse Imaging in Near-infrared” (DOLPHIN), that resolves these challenges. DOLPHIN achieves the following: (i) resolution of probes through up to 8 cm of tissue phantom; (ii) identifcation of spectral and scattering signatures of tissues without a priori knowledge of background or autofuorescence; and (iii) 3D reconstruction of live whole animals. Notably, we demonstrate noninvasive real-time tracking of a 0.1 mm-sized fuorophore through the gastrointestinal tract of a living mouse, which is beyond the detection limit of current imaging modalities. |
| first_indexed | 2024-09-23T15:51:16Z |
| format | Article |
| id | mit-1721.1/121128 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:51:16Z |
| publishDate | 2019 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1211282022-10-02T04:38:03Z Deep-tissue optical imaging of near cellular-sized features Dang, Xiangnan Bardhan, Neelkanth Manoj Qi, Jifa Gu, Li Eze, Ngozi A Lin, Ching-Wei Kataria, Swati Hammond, Paula T Belcher, Angela M Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Koch Institute for Integrative Cancer Research at MIT Bardhan, Neelkanth Dang, Xiangnan Bardhan, Neelkanth Manoj Qi, Jifa Gu, Li Eze, Ngozi A Lin, Ching-Wei Kataria, Swati Hammond, Paula T Belcher, Angela M Detection of biological features at the cellular level with sufcient sensitivity in complex tissue remains a major challenge. To appreciate this challenge, this would require fnding tens to hundreds of cells (a 0.1 mm tumor has ~125 cells), out of ~37 trillion cells in the human body. Near-infrared optical imaging holds promise for high-resolution, deep-tissue imaging, but is limited by autofuorescence and scattering. To date, the maximum reported depth using second-window near-infrared (NIR-II: 1000–1700 nm) fuorophores is 3.2 cm through tissue. Here, we design an NIR-II imaging system, “Detection of Optically Luminescent Probes using Hyperspectral and difuse Imaging in Near-infrared” (DOLPHIN), that resolves these challenges. DOLPHIN achieves the following: (i) resolution of probes through up to 8 cm of tissue phantom; (ii) identifcation of spectral and scattering signatures of tissues without a priori knowledge of background or autofuorescence; and (iii) 3D reconstruction of live whole animals. Notably, we demonstrate noninvasive real-time tracking of a 0.1 mm-sized fuorophore through the gastrointestinal tract of a living mouse, which is beyond the detection limit of current imaging modalities. Untied States. National Cancer Institute. Cancer Center Support (Grant P30-CA14051) United States. National Cancer Institute. Center for Cancer Nanotechnology Excellence (Grant 5-U54-CA151884-03) 2019-03-29T21:39:15Z 2019-03-29T21:39:15Z 2019-03 2017-12 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/121128 Dang, Xiangnan et al. “Deep-Tissue Optical Imaging of Near Cellular-Sized Features.” Scientific Reports 9, 1 (March 2019). doi:10.1038/s41598-019-39502-w. © 2019 The Author(s) https://orcid.org/0000-0002-4343-4007 https://orcid.org/0000-0002-7530-4725 https://orcid.org/0000-0001-5646-1007 https://orcid.org/0000-0002-4171-3547 https://orcid.org/0000-0001-9353-7453 en_US https://doi.org/10.1038/s41598-019-39502-w Scientific Reports Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Scientific Reports |
| spellingShingle | Dang, Xiangnan Bardhan, Neelkanth Manoj Qi, Jifa Gu, Li Eze, Ngozi A Lin, Ching-Wei Kataria, Swati Hammond, Paula T Belcher, Angela M Deep-tissue optical imaging of near cellular-sized features |
| title | Deep-tissue optical imaging of near cellular-sized features |
| title_full | Deep-tissue optical imaging of near cellular-sized features |
| title_fullStr | Deep-tissue optical imaging of near cellular-sized features |
| title_full_unstemmed | Deep-tissue optical imaging of near cellular-sized features |
| title_short | Deep-tissue optical imaging of near cellular-sized features |
| title_sort | deep tissue optical imaging of near cellular sized features |
| url | http://hdl.handle.net/1721.1/121128 https://orcid.org/0000-0002-4343-4007 https://orcid.org/0000-0002-7530-4725 https://orcid.org/0000-0001-5646-1007 https://orcid.org/0000-0002-4171-3547 https://orcid.org/0000-0001-9353-7453 |
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