Directing curli polymerization with DNA origami nucleators
The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorate...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019
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| Online Access: | https://hdl.handle.net/1721.1/121274 |
| _version_ | 1826203816729509888 |
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| author | Mao, Xiuhai Li, Ke Liu, Mengmeng Wang, Xinyu Zhao, Tianxin An, Bolin Cui, Mengkui Li, Yingfeng Pu, Jiahua Li, Jiang Wang, Lihua Lu, Timothy K Fan, Chunhai Zhong, Chao |
| author2 | Massachusetts Institute of Technology. Synthetic Biology Center |
| author_facet | Massachusetts Institute of Technology. Synthetic Biology Center Mao, Xiuhai Li, Ke Liu, Mengmeng Wang, Xinyu Zhao, Tianxin An, Bolin Cui, Mengkui Li, Yingfeng Pu, Jiahua Li, Jiang Wang, Lihua Lu, Timothy K Fan, Chunhai Zhong, Chao |
| author_sort | Mao, Xiuhai |
| collection | MIT |
| description | The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and then accelerates fibril formation by increasing the proliferation rate of primary pathways. Fibrils grow either out from (departure mode) or towards the nucleators (arrival mode), implying two distinct roles of CsgB: as nucleation sites and as trap sites to capture growing nanofibrils in vicinity. Curli polymerization follows typical stop-and-go dynamics but exhibits a higher instantaneous elongation rate compared with independent fibril growth. This origami nucleator thus provides an in vitro platform for mechanistically probing molecular nucleation and controlling directional fibril polymerization for bionanotechnology. |
| first_indexed | 2024-09-23T12:44:00Z |
| format | Article |
| id | mit-1721.1/121274 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:44:00Z |
| publishDate | 2019 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1212742022-09-28T09:42:47Z Directing curli polymerization with DNA origami nucleators Mao, Xiuhai Li, Ke Liu, Mengmeng Wang, Xinyu Zhao, Tianxin An, Bolin Cui, Mengkui Li, Yingfeng Pu, Jiahua Li, Jiang Wang, Lihua Lu, Timothy K Fan, Chunhai Zhong, Chao Massachusetts Institute of Technology. Synthetic Biology Center Massachusetts Institute of Technology. Research Laboratory of Electronics The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and then accelerates fibril formation by increasing the proliferation rate of primary pathways. Fibrils grow either out from (departure mode) or towards the nucleators (arrival mode), implying two distinct roles of CsgB: as nucleation sites and as trap sites to capture growing nanofibrils in vicinity. Curli polymerization follows typical stop-and-go dynamics but exhibits a higher instantaneous elongation rate compared with independent fibril growth. This origami nucleator thus provides an in vitro platform for mechanistically probing molecular nucleation and controlling directional fibril polymerization for bionanotechnology. 2019-06-13T21:42:12Z 2019-06-13T21:42:12Z 2019-03 2018-04 2019-06-13T14:08:50Z Article http://purl.org/eprint/type/JournalArticle 2041-1723 https://hdl.handle.net/1721.1/121274 Mao, Xiuhai et al. "Directing curli polymerization with DNA origami nucleators." Nature Communications 10 (March 2019): 1395 © 2019 The Author(s) en http://dx.doi.org/10.1038/s41467-019-09369-6 Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Mao, Xiuhai Li, Ke Liu, Mengmeng Wang, Xinyu Zhao, Tianxin An, Bolin Cui, Mengkui Li, Yingfeng Pu, Jiahua Li, Jiang Wang, Lihua Lu, Timothy K Fan, Chunhai Zhong, Chao Directing curli polymerization with DNA origami nucleators |
| title | Directing curli polymerization with DNA origami nucleators |
| title_full | Directing curli polymerization with DNA origami nucleators |
| title_fullStr | Directing curli polymerization with DNA origami nucleators |
| title_full_unstemmed | Directing curli polymerization with DNA origami nucleators |
| title_short | Directing curli polymerization with DNA origami nucleators |
| title_sort | directing curli polymerization with dna origami nucleators |
| url | https://hdl.handle.net/1721.1/121274 |
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