Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues
Three-dimensional (3D) tissue imaging methods are expected to improve surgical management of cancer. In this study, we examined the feasibility of two 3D imaging technologies, optical coherence tomography (OCT) and optical coherence microscopy (OCM), to view human breast specimens based on intrinsic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for Cancer Research (AACR)
2019
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| Online Access: | https://hdl.handle.net/1721.1/121501 |
| _version_ | 1811068882848317440 |
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| author | Zhou, Chao Cohen, David W. Wang, Yihong Lee, Hsiang-Chieh Mondelblatt, Amy E. Tsai, Tsung-Han Aguirre, Aaron Dominic Fujimoto, James G Connolly, James L. |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Zhou, Chao Cohen, David W. Wang, Yihong Lee, Hsiang-Chieh Mondelblatt, Amy E. Tsai, Tsung-Han Aguirre, Aaron Dominic Fujimoto, James G Connolly, James L. |
| author_sort | Zhou, Chao |
| collection | MIT |
| description | Three-dimensional (3D) tissue imaging methods are expected to improve surgical management of cancer. In this study, we examined the feasibility of two 3D imaging technologies, optical coherence tomography (OCT) and optical coherence microscopy (OCM), to view human breast specimens based on intrinsic optical contrast. Specifically, we imaged 44 ex vivo breast specimens including 34 benign and 10 malignant lesions with an integrated OCT and OCM system developed in our laboratory. The system enabled 4-μm axial resolution (OCT and OCM) with 14-μm (OCT) and 2-μm (OCM) transverse resolutions, respectively. OCT and OCM images were compared with corresponding histologic sections to identify characteristic features from benign and malignant breast lesions at multiple resolution scales. OCT and OCM provide complimentary information about tissue microstructure, thus showing distinctive patterns for adipose tissue, fibrous stroma, breast lobules and ducts, cysts and microcysts, as well as in situ and invasive carcinomas. The 3D imaging capability of OCT and OCM provided complementary information to individual 2D images, thereby allowing tracking features from different levels to identify low-contrast structures that were difficult to appreciate from single images alone. Our results lay the foundation for future in vivo optical evaluation of breast tissues, using OCT and OCM, which has the potential to guide core needle biopsies, assess surgical margins, and evaluate nodal involvement in breast cancer. |
| first_indexed | 2024-09-23T08:02:27Z |
| format | Article |
| id | mit-1721.1/121501 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:02:27Z |
| publishDate | 2019 |
| publisher | American Association for Cancer Research (AACR) |
| record_format | dspace |
| spelling | mit-1721.1/1215012022-09-30T01:54:12Z Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues Zhou, Chao Cohen, David W. Wang, Yihong Lee, Hsiang-Chieh Mondelblatt, Amy E. Tsai, Tsung-Han Aguirre, Aaron Dominic Fujimoto, James G Connolly, James L. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Three-dimensional (3D) tissue imaging methods are expected to improve surgical management of cancer. In this study, we examined the feasibility of two 3D imaging technologies, optical coherence tomography (OCT) and optical coherence microscopy (OCM), to view human breast specimens based on intrinsic optical contrast. Specifically, we imaged 44 ex vivo breast specimens including 34 benign and 10 malignant lesions with an integrated OCT and OCM system developed in our laboratory. The system enabled 4-μm axial resolution (OCT and OCM) with 14-μm (OCT) and 2-μm (OCM) transverse resolutions, respectively. OCT and OCM images were compared with corresponding histologic sections to identify characteristic features from benign and malignant breast lesions at multiple resolution scales. OCT and OCM provide complimentary information about tissue microstructure, thus showing distinctive patterns for adipose tissue, fibrous stroma, breast lobules and ducts, cysts and microcysts, as well as in situ and invasive carcinomas. The 3D imaging capability of OCT and OCM provided complementary information to individual 2D images, thereby allowing tracking features from different levels to identify low-contrast structures that were difficult to appreciate from single images alone. Our results lay the foundation for future in vivo optical evaluation of breast tissues, using OCT and OCM, which has the potential to guide core needle biopsies, assess surgical margins, and evaluate nodal involvement in breast cancer. National Institutes of Health (U.S.) (Grant R01-CA75289-13) Air Force Office of Scientific Research (Contract FA9550-07-1-0014) 2019-07-05T19:00:05Z 2019-07-05T19:00:05Z 2010-11 2010-10 2019-06-21T17:04:13Z Article http://purl.org/eprint/type/JournalArticle 0008-5472 1538-7445 https://hdl.handle.net/1721.1/121501 Zhou, Chao et al. "Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues." Cancer Research 70, 24 (December 2010): 10071-10079 © 2010 American Association for Cancer Research (AACR) en https://doi.org/10.1158/0008-5472.CAN-10-2968 Cancer Research Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Association for Cancer Research (AACR) PMC |
| spellingShingle | Zhou, Chao Cohen, David W. Wang, Yihong Lee, Hsiang-Chieh Mondelblatt, Amy E. Tsai, Tsung-Han Aguirre, Aaron Dominic Fujimoto, James G Connolly, James L. Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues |
| title | Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues |
| title_full | Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues |
| title_fullStr | Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues |
| title_full_unstemmed | Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues |
| title_short | Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues |
| title_sort | integrated optical coherence tomography and microscopy for ex vivo multiscale evaluation of human breast tissues |
| url | https://hdl.handle.net/1721.1/121501 |
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