Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood
In blood samples from patients with viral infection, it is often important to separate viral particles from human cells, for example, to minimize background in performing viral whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics with continuous cir...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society (ACS)
2019
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| Online Access: | https://hdl.handle.net/1721.1/121535 |
| _version_ | 1826208817917984768 |
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| author | Choi, Kyungyong Ryu, Hyunryul Siddle, Katherine J. Piantadosi, Anne Freimark, Lisa Park, Daniel J. Sabeti, Pardis Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Choi, Kyungyong Ryu, Hyunryul Siddle, Katherine J. Piantadosi, Anne Freimark, Lisa Park, Daniel J. Sabeti, Pardis Han, Jongyoon |
| author_sort | Choi, Kyungyong |
| collection | MIT |
| description | In blood samples from patients with viral infection, it is often important to separate viral particles from human cells, for example, to minimize background in performing viral whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics with continuous circulation to separate host cells from viral particles and free nucleic acid. We demonstrate that this device effectively reduces white blood cells, red blood cells, and platelets from both whole blood and plasma samples with excellent recovery of viral nucleic acid. Furthermore, microfluidic separation leads to greater viral genome coverage and depth, highlighting an important application of this device in processing clinical samples for viral genome sequencing. |
| first_indexed | 2024-09-23T14:12:49Z |
| format | Article |
| id | mit-1721.1/121535 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:12:49Z |
| publishDate | 2019 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1215352022-10-01T19:50:21Z Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood Choi, Kyungyong Ryu, Hyunryul Siddle, Katherine J. Piantadosi, Anne Freimark, Lisa Park, Daniel J. Sabeti, Pardis Han, Jongyoon Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Research Laboratory of Electronics In blood samples from patients with viral infection, it is often important to separate viral particles from human cells, for example, to minimize background in performing viral whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics with continuous circulation to separate host cells from viral particles and free nucleic acid. We demonstrate that this device effectively reduces white blood cells, red blood cells, and platelets from both whole blood and plasma samples with excellent recovery of viral nucleic acid. Furthermore, microfluidic separation leads to greater viral genome coverage and depth, highlighting an important application of this device in processing clinical samples for viral genome sequencing. National Institutes of Health (U.S.) (R01AI117043) National Institutes of Health (U.S.) (U24-AI118656) Broad Institute 2019-07-09T14:46:38Z 2019-07-09T14:46:38Z 2018-03-14 2019-06-05T14:59:51Z Article http://purl.org/eprint/type/JournalArticle 0003-2700 1520-6882 https://hdl.handle.net/1721.1/121535 Choi, Kyungyong, et al. “Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood.” Analytical Chemistry 90, no. 7 (April 2018): 4657–62. en 10.1021/acs.analchem.7b05200 Analytical Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Choi, Kyungyong Ryu, Hyunryul Siddle, Katherine J. Piantadosi, Anne Freimark, Lisa Park, Daniel J. Sabeti, Pardis Han, Jongyoon Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood |
| title | Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood |
| title_full | Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood |
| title_fullStr | Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood |
| title_full_unstemmed | Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood |
| title_short | Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood |
| title_sort | negative selection by spiral inertial microfluidics improves viral recovery and sequencing from blood |
| url | https://hdl.handle.net/1721.1/121535 |
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