Towards brain-wide noninvasive molecular imaging
Thesis: Ph. D. in Medical Engineering and Medical Physics, Harvard-MIT Program in Health Sciences and Technology, 2019
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2019
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| Online Access: | https://hdl.handle.net/1721.1/122128 |
| _version_ | 1811088558062043136 |
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| author | Wiśniowska, Agata Elżbieta. |
| author2 | Alan P. Jasanoff. |
| author_facet | Alan P. Jasanoff. Wiśniowska, Agata Elżbieta. |
| author_sort | Wiśniowska, Agata Elżbieta. |
| collection | MIT |
| description | Thesis: Ph. D. in Medical Engineering and Medical Physics, Harvard-MIT Program in Health Sciences and Technology, 2019 |
| first_indexed | 2024-09-23T14:04:03Z |
| format | Thesis |
| id | mit-1721.1/122128 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:04:03Z |
| publishDate | 2019 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1221282019-09-19T03:02:45Z Towards brain-wide noninvasive molecular imaging Wiśniowska, Agata Elżbieta. Alan P. Jasanoff. Harvard--MIT Program in Health Sciences and Technology. Harvard--MIT Program in Health Sciences and Technology Harvard--MIT Program in Health Sciences and Technology. Thesis: Ph. D. in Medical Engineering and Medical Physics, Harvard-MIT Program in Health Sciences and Technology, 2019 Cataloged from PDF version of thesis. Includes bibliographical references. An intricate interplay of signaling molecules underlies brain activity, yet studying these molecular events in living whole organisms remains a challenge. Magnetic resonance imaging (MRI) is the most promising imaging modality for development of molecular signaling sensors with deeper tissue penetration than optical imaging, and better spatial resolution and more dynamic potential in sensor design, compared to radioactive probes. MRI molecular sensors, however, have largely required micromolar concentrations to achieve detectable signals. In order to detect signaling molecules in the brain at their native low nanomolar concentrations, an improvement in MRI molecular sensors is necessary. Here we introduce a new in vivo imaging paradigm that uses vasoactive probes (vasoprobes) that couple molecular signals to vascular responses. We apply the vasoprobes to detect molecular targets at nanomolar concentrations in living rodent brains, thus satisfying the sensitivity requirement for imaging endogenous signaling events. Even with more sensitive probes, molecular imaging of the brain is further complicated by the presence of the blood-brain barrier (BBB), designed by nature to protect this most vital of organs. We have therefore implemented a means to permit noninvasive delivery of imaging agents following ultrasonic BBB opening. We use the ultrasound technique to deliver another potent class of contrast agents, superparamagnetic iron oxides, and we show that effective permeation of brain tissue is achieved using this approach. We have also designed ultrasensitive vasoprobe variants designed to permeate the brain completely noninvasively, using endogenous transporter-mediated mechanisms. We present preliminary results based on this approach and discuss future directions. by Agata E. Wiśniowska. Ph. D. in Medical Engineering and Medical Physics Ph.D.inMedicalEngineeringandMedicalPhysics Harvard-MIT Program in Health Sciences and Technology 2019-09-16T20:59:44Z 2019-09-16T20:59:44Z 2019 2019 Thesis https://hdl.handle.net/1721.1/122128 1119539081 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 77 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Harvard--MIT Program in Health Sciences and Technology. Wiśniowska, Agata Elżbieta. Towards brain-wide noninvasive molecular imaging |
| title | Towards brain-wide noninvasive molecular imaging |
| title_full | Towards brain-wide noninvasive molecular imaging |
| title_fullStr | Towards brain-wide noninvasive molecular imaging |
| title_full_unstemmed | Towards brain-wide noninvasive molecular imaging |
| title_short | Towards brain-wide noninvasive molecular imaging |
| title_sort | towards brain wide noninvasive molecular imaging |
| topic | Harvard--MIT Program in Health Sciences and Technology. |
| url | https://hdl.handle.net/1721.1/122128 |
| work_keys_str_mv | AT wisniowskaagataelzbieta towardsbrainwidenoninvasivemolecularimaging |