Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2019
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2019
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| Online Access: | https://hdl.handle.net/1721.1/122531 |
| _version_ | 1811072553387556864 |
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| author | Uranukul, Boonsom. |
| author2 | Gregory Stephanopoulos. |
| author_facet | Gregory Stephanopoulos. Uranukul, Boonsom. |
| author_sort | Uranukul, Boonsom. |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2019 |
| first_indexed | 2024-09-23T09:07:45Z |
| format | Thesis |
| id | mit-1721.1/122531 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:07:45Z |
| publishDate | 2019 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1225312019-11-22T03:40:04Z Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass Uranukul, Boonsom. Gregory Stephanopoulos. Massachusetts Institute of Technology. Department of Chemical Engineering. Massachusetts Institute of Technology. Department of Chemical Engineering Chemical Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2019 Cataloged from PDF version of thesis. Includes bibliographical references. Currently, plastics are almost exclusively produced from feedstocks derived from crude oil refining and natural gas processing. Despite the increasing awareness of the negative environmental and climate-related impacts associated with fossil fuel consumption, the relevance of fossil fuels has held steady as a result of the recent proliferation of plastics industry. The recent growing attempts to replace conventional petroleum-based production processes with renewable direct bioconversion processes, however, have not yet been made successful due to low production efficiency. Here, we studied the development of a bioprocess for the renewable production of monoethylene glycol (MEG), a precursor chemical of polyethylene terephthalate (PET) plastics, by using yeast Saccharomyces cerevisiae as biosynthesis platform. During the process, we found evidence for the existence of an endogenous biosynthetic route for MEG production from D-xylose in S. cerevisiae. Based on the discovered biosynthetic pathway, we then demonstrated the implementation of metabolic engineering and fermentation operational strategies that led to an overproduction of MEG, as well as improved strain performance during prolonged bioreactor cultivation. Using the MEG bioconversion process as the starting point, we developed another bioprocess which allowed direct conversion of D-xylose to glycolic acid, a chemical precursor of polylactic-co-glycolic acid (PLGA). Furthermore, we investigated the biosynthesis of 1,4- butanediol, a chemical precursor of thermoplastic engineering polymer polybutylene terephthalate (PBT), in S. cerevisiae. In all of these studies, ethanol fermentation emerged as an important limitation that negatively affected the efficiency of the yeast-based processes. Our attempts to disrupt ethanol fermentation, while successfully reducing ethanol production, led to a compromise in MEG production. An analysis on the energetics of our engineered S. cerevisiae, revealed that ethanol fermentation might, in fact, be a necessary requirement for maintaining the energy balance in certain systems, including the biosynthesis of MEG. These findings provide some insights as well as a better understanding of Saccharonyces cerevisiae as the microbial cell factory for the biosynthesis of small molecules other than ethanol. by Boonsom Uranukul. Ph. D. Ph.D. Massachusetts Institute of Technology, Department of Chemical Engineering 2019-10-11T22:00:34Z 2019-10-11T22:00:34Z 2019 2019 Thesis https://hdl.handle.net/1721.1/122531 1121594768 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 220 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemical Engineering. Uranukul, Boonsom. Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| title | Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| title_full | Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| title_fullStr | Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| title_full_unstemmed | Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| title_short | Engineering of Saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| title_sort | engineering of saccharomyces cerevisiae for renewable production of plastic precursor chemicals from plant biomass |
| topic | Chemical Engineering. |
| url | https://hdl.handle.net/1721.1/122531 |
| work_keys_str_mv | AT uranukulboonsom engineeringofsaccharomycescerevisiaeforrenewableproductionofplasticprecursorchemicalsfromplantbiomass |