Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques
Enterococcus faecalis is a common opportunistic pathogen that colonizes cephalic recording chambers (CRCs) of macaques used in cognitive neuroscience research. We previously characterized 15 E. faecalis strains isolated from macaques at the Massachusetts Institute of Technology (MIT) in 2011. The go...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019
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| Online Access: | https://hdl.handle.net/1721.1/122645 |
| _version_ | 1826192516240637952 |
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| author | Lieberman, Mia Van Tyne, Daria Dzink-Fox, JoAnn Ma, Eric J. Gilmore, Michael S. Fox, James G |
| author2 | Massachusetts Institute of Technology. Division of Comparative Medicine |
| author_facet | Massachusetts Institute of Technology. Division of Comparative Medicine Lieberman, Mia Van Tyne, Daria Dzink-Fox, JoAnn Ma, Eric J. Gilmore, Michael S. Fox, James G |
| author_sort | Lieberman, Mia |
| collection | MIT |
| description | Enterococcus faecalis is a common opportunistic pathogen that colonizes cephalic recording chambers (CRCs) of macaques used in cognitive neuroscience research. We previously characterized 15 E. faecalis strains isolated from macaques at the Massachusetts Institute of Technology (MIT) in 2011. The goal of this study was to examine how a 2014 protocol change prohibiting the use of antimicrobials within CRCs affected colonizing E. faecalis strains. We collected 20 E. faecalis isolates from 10 macaques between 2013 and 2017 for comparison to 4 isolates previously characterized in 2011 with respect to the sequence type (ST) distribution, antimicrobial resistance, biofilm formation, and changes in genes that might confer a survival advantage. ST4 and ST55 were predominant among the isolates characterized in 2011, whereas the less antimicrobial-resistant lineage ST48 emerged to dominance after 2013. Two macaques remained colonized by ST4 and ST55 strains for 5 and 4 years, respectively. While the antimicrobial resistance and virulence factors identified in these ST4 and ST55 strains remained relatively stable, we detected an increase in biofilm formation ability over time in both isolates. We also found that ST48 strains were typically robust biofilm formers, which could explain why this ST increased in prevalence. Finally, we identified mutations in the DNA mismatch repair genes mutS and mutL in separate ST55 and ST4 strains and confirmed that strains bearing these mutations displayed a hypermutator phenotype. The presence of a hypermutator phenotype may complicate future antimicrobial treatment for clinically relevant E. faecalis infections in macaques. |
| first_indexed | 2024-09-23T09:18:21Z |
| format | Article |
| id | mit-1721.1/122645 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:18:21Z |
| publishDate | 2019 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | mit-1721.1/1226452022-09-26T11:14:08Z Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques Lieberman, Mia Van Tyne, Daria Dzink-Fox, JoAnn Ma, Eric J. Gilmore, Michael S. Fox, James G Massachusetts Institute of Technology. Division of Comparative Medicine Massachusetts Institute of Technology. Department of Biological Engineering Enterococcus faecalis is a common opportunistic pathogen that colonizes cephalic recording chambers (CRCs) of macaques used in cognitive neuroscience research. We previously characterized 15 E. faecalis strains isolated from macaques at the Massachusetts Institute of Technology (MIT) in 2011. The goal of this study was to examine how a 2014 protocol change prohibiting the use of antimicrobials within CRCs affected colonizing E. faecalis strains. We collected 20 E. faecalis isolates from 10 macaques between 2013 and 2017 for comparison to 4 isolates previously characterized in 2011 with respect to the sequence type (ST) distribution, antimicrobial resistance, biofilm formation, and changes in genes that might confer a survival advantage. ST4 and ST55 were predominant among the isolates characterized in 2011, whereas the less antimicrobial-resistant lineage ST48 emerged to dominance after 2013. Two macaques remained colonized by ST4 and ST55 strains for 5 and 4 years, respectively. While the antimicrobial resistance and virulence factors identified in these ST4 and ST55 strains remained relatively stable, we detected an increase in biofilm formation ability over time in both isolates. We also found that ST48 strains were typically robust biofilm formers, which could explain why this ST increased in prevalence. Finally, we identified mutations in the DNA mismatch repair genes mutS and mutL in separate ST55 and ST4 strains and confirmed that strains bearing these mutations displayed a hypermutator phenotype. The presence of a hypermutator phenotype may complicate future antimicrobial treatment for clinically relevant E. faecalis infections in macaques. National Institutes of Health (U.S.) (Grant T32-OD010978) National Institutes of Health (U.S.) (Grant P30-ES002109) 2019-10-21T15:50:10Z 2019-10-21T15:50:10Z 2018-07 2018-06 2019-10-17T13:19:09Z Article http://purl.org/eprint/type/JournalArticle 0099-2240 1098-5336 https://hdl.handle.net/1721.1/122645 Lieberman, Mia T. et al. "Long-Term Colonization Dynamics of Enterococcus faecalis in Implanted Devices in Research Macaques." Applied and Environmental Microbiology 84, 18 (July 2018): e01336-18 © 2018 American Society for Microbiology en http://dx.doi.org/10.1128/aem.01336-18 Applied and Environmental Microbiology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Society for Microbiology Prof. Fox via Howard Silver |
| spellingShingle | Lieberman, Mia Van Tyne, Daria Dzink-Fox, JoAnn Ma, Eric J. Gilmore, Michael S. Fox, James G Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques |
| title | Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques |
| title_full | Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques |
| title_fullStr | Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques |
| title_full_unstemmed | Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques |
| title_short | Long-Term Colonization Dynamics ofEnterococcus faecalisin Implanted Devices in Research Macaques |
| title_sort | long term colonization dynamics ofenterococcus faecalisin implanted devices in research macaques |
| url | https://hdl.handle.net/1721.1/122645 |
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