Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus
Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2018
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2019
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| Online Access: | https://hdl.handle.net/1721.1/122875 |
| _version_ | 1826195514690895872 |
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| author | Serrano, Jean Carlos. |
| author2 | Roger D. Kamm. |
| author_facet | Roger D. Kamm. Serrano, Jean Carlos. |
| author_sort | Serrano, Jean Carlos. |
| collection | MIT |
| description | Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2018 |
| first_indexed | 2024-09-23T10:13:57Z |
| format | Thesis |
| id | mit-1721.1/122875 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T10:13:57Z |
| publishDate | 2019 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1228752019-11-22T03:30:39Z Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus Engineering 3D lymphatic vasculature on-chip through biochemical and mechanical stimulus Serrano, Jean Carlos. Roger D. Kamm. Massachusetts Institute of Technology. Department of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering Mechanical Engineering. Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2018 Cataloged from PDF version of thesis. Includes bibliographical references (pages 50-55). In this thesis, we developed a microfluidic-based platform for the generation of physiologically relevant 3D lymphatic capillaries. This tissue engineering platform allowed us to probe the vascularization dynamics of lymphatic endothelial cells under a highly controlled microenvironment and isolate the effects of biochemical and biophysical inputs. Under this precise control over local extracellular factors, we studied the angiogenic response of lymphatic endothelial cells to different soluble pro-angiogenic factors, which accordingly induced different sprout formation dynamics . We also controlled the vascularization behaviors of lymphatics by modulating the intrinsic composition of the extracellular matrix. Finally, we explored the influence of mechanical stimuli, more specifically interstitial flow, on the formation of lymphatic sprouts to which we observe a dependency on the synergistic stimulus from the presence of pro-angiogenic factors while inducing interstitial flow. In summary, these results elucidate the physiological process of lymphatic angiogenesis and explores the individual contribution of local cues in the cellular microenvironment during this vascular morphogenesis phenomenon. Additionally, the development of this platform has potential applications for physiological studies regarding lymphatic function, regenerative medicine and drug development for lymphatic-associated diseases.. by Jean Carlos Serrano S.M. S.M. Massachusetts Institute of Technology, Department of Mechanical Engineering 2019-11-12T17:40:50Z 2019-11-12T17:40:50Z 2018 2018 Thesis https://hdl.handle.net/1721.1/122875 1126653880 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 63 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Serrano, Jean Carlos. Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus |
| title | Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus |
| title_full | Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus |
| title_fullStr | Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus |
| title_full_unstemmed | Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus |
| title_short | Engineering three-dimensional lymphatic vasculature on-chip through biochemical and mechanical stimulus |
| title_sort | engineering three dimensional lymphatic vasculature on chip through biochemical and mechanical stimulus |
| topic | Mechanical Engineering. |
| url | https://hdl.handle.net/1721.1/122875 |
| work_keys_str_mv | AT serranojeancarlos engineeringthreedimensionallymphaticvasculatureonchipthroughbiochemicalandmechanicalstimulus AT serranojeancarlos engineering3dlymphaticvasculatureonchipthroughbiochemicalandmechanicalstimulus |