Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging
Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology. Those probes that respond to enzymatic catalysis illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and s...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2020
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| Online Access: | https://hdl.handle.net/1721.1/123655 |
| _version_ | 1826199813143658496 |
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| author | Chyan, Wen Raines, Ronald T |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Chyan, Wen Raines, Ronald T |
| author_sort | Chyan, Wen |
| collection | MIT |
| description | Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology. Those probes that respond to enzymatic catalysis illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. Here, we review recent advances in enzyme-activated fluorogenic probes for biological imaging. We organize our survey by enzyme classification, with emphasis on fluorophore masking strategies, modes of enzymatic activation, and the breadth of current and future applications. Key challenges such as probe selectivity and spectroscopic requirements are described alongside therapeutic, diagnostic, and theranostic opportunities. Keywords: cells; peptides and proteins; imaging probes; fluorescence; probes |
| first_indexed | 2024-09-23T11:26:27Z |
| format | Article |
| id | mit-1721.1/123655 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:26:27Z |
| publishDate | 2020 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1236552022-09-27T19:32:13Z Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging Chyan, Wen Raines, Ronald T Massachusetts Institute of Technology. Department of Chemistry Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology. Those probes that respond to enzymatic catalysis illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. Here, we review recent advances in enzyme-activated fluorogenic probes for biological imaging. We organize our survey by enzyme classification, with emphasis on fluorophore masking strategies, modes of enzymatic activation, and the breadth of current and future applications. Key challenges such as probe selectivity and spectroscopic requirements are described alongside therapeutic, diagnostic, and theranostic opportunities. Keywords: cells; peptides and proteins; imaging probes; fluorescence; probes National Institutes of Health (U.S.) (Grant R01 GM044783) National Institutes of Health (U.S.) (Grant R01 CA073808) 2020-01-23T19:27:45Z 2020-01-23T19:27:45Z 2018-06 2018-04 2020-01-07T14:22:59Z Article http://purl.org/eprint/type/JournalArticle 1554-8929 1554-8937 https://hdl.handle.net/1721.1/123655 Chyan, Wen and Ronald T. Raines. "Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging." ACS Chemical Biology 13, 7 (June 2018): 1810-1823 © 2018 American Chemical Society en http://dx.doi.org/10.1021/acschembio.8b00371 ACS Chemical Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Chyan, Wen Raines, Ronald T Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging |
| title | Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging |
| title_full | Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging |
| title_fullStr | Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging |
| title_full_unstemmed | Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging |
| title_short | Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging |
| title_sort | enzyme activated fluorogenic probes for live cell and in vivo imaging |
| url | https://hdl.handle.net/1721.1/123655 |
| work_keys_str_mv | AT chyanwen enzymeactivatedfluorogenicprobesforlivecellandinvivoimaging AT rainesronaldt enzymeactivatedfluorogenicprobesforlivecellandinvivoimaging |