Affinity-based capture and identification of protein effectors of the growth regulator ppGpp

The nucleotide ppGpp is a highly conserved regulatory molecule in bacteria that helps tune growth rate to nutrient availability. Despite decades of study, how ppGpp regulates growth remains poorly understood. Here, we developed and validated a capture-compound mass spectrometry approach that identif...

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Bibliographic Details
Main Authors: Wang, Boyuan, Dai, Peng, Ding, David, Del Rosario, Amanda M, Grant, Robert A, Pentelute, Bradley L., Laub, Michael T
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2020
Online Access:https://hdl.handle.net/1721.1/123670