A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells
The MS2 system is a powerful tool for investigating transcription dynamics at the single molecule directly in live cells. In the past, insertion of the RNA-labelling cassette at specific gene loci has been a major hurdle. Here, we present a CRISPR/Cas9-based approach to insert an MS2 cassette with s...
| Main Authors: | , , , , , |
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| 其他作者: | |
| 格式: | 文件 |
| 语言: | English |
| 出版: |
Elsevier BV
2020
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| 在线阅读: | https://hdl.handle.net/1721.1/123695 |