Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivato...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2020
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| Online Access: | https://hdl.handle.net/1721.1/123696 |
| _version_ | 1811098031109439488 |
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| author | Cho, Won-ki Spille, Jan Hendrik Hecht, Micca Lee, Choongman Li, Charles Han Grube, Valentin Cisse, Ibrahim I |
| author2 | Massachusetts Institute of Technology. Department of Physics |
| author_facet | Massachusetts Institute of Technology. Department of Physics Cho, Won-ki Spille, Jan Hendrik Hecht, Micca Lee, Choongman Li, Charles Han Grube, Valentin Cisse, Ibrahim I |
| author_sort | Cho, Won-ki |
| collection | MIT |
| description | Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo. |
| first_indexed | 2024-09-23T17:08:52Z |
| format | Article |
| id | mit-1721.1/123696 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:08:52Z |
| publishDate | 2020 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1236962022-09-29T23:59:02Z Mediator and RNA polymerase II clusters associate in transcription-dependent condensates Cho, Won-ki Spille, Jan Hendrik Hecht, Micca Lee, Choongman Li, Charles Han Grube, Valentin Cisse, Ibrahim I Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. Department of Biology MIT Materials Research Laboratory Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo. National Institutes of Health (U.S.) (Grant DP2CA195769) 2020-02-05T20:50:27Z 2020-02-05T20:50:27Z 2018 2018 2020-01-21T15:11:37Z Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 https://hdl.handle.net/1721.1/123696 Cho, Won-Ki et al. "Mediator and RNA polymerase II clusters associate in transcription-dependent condensates." Science 361, 6400 (July 2018): 412-415 © 2018 Academic Press en http://dx.doi.org/10.1126/science.aar4199 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) Prof. Spille via Barbara Williams |
| spellingShingle | Cho, Won-ki Spille, Jan Hendrik Hecht, Micca Lee, Choongman Li, Charles Han Grube, Valentin Cisse, Ibrahim I Mediator and RNA polymerase II clusters associate in transcription-dependent condensates |
| title | Mediator and RNA polymerase II clusters associate in transcription-dependent condensates |
| title_full | Mediator and RNA polymerase II clusters associate in transcription-dependent condensates |
| title_fullStr | Mediator and RNA polymerase II clusters associate in transcription-dependent condensates |
| title_full_unstemmed | Mediator and RNA polymerase II clusters associate in transcription-dependent condensates |
| title_short | Mediator and RNA polymerase II clusters associate in transcription-dependent condensates |
| title_sort | mediator and rna polymerase ii clusters associate in transcription dependent condensates |
| url | https://hdl.handle.net/1721.1/123696 |
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