Radical polymerization reactions for amplified biodetection signals
Chemical reactions that provide amplified biodetection signals are essential in point-of-care diagnostics, a category of portable biosensors that should detect nanomolar to attomolar concentrations of clinically actionable biomarkers in bodily fluids without using advanced lab equipment. As an alter...
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| Format: | Article |
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Royal Society of Chemistry (RSC)
2020
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| Online Access: | https://hdl.handle.net/1721.1/123795 |
| _version_ | 1826199302898188288 |
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| author | Kim, Seunghyeon Sikes Johnson, Hadley |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Kim, Seunghyeon Sikes Johnson, Hadley |
| author_sort | Kim, Seunghyeon |
| collection | MIT |
| description | Chemical reactions that provide amplified biodetection signals are essential in point-of-care diagnostics, a category of portable biosensors that should detect nanomolar to attomolar concentrations of clinically actionable biomarkers in bodily fluids without using advanced lab equipment. As an alternative to common signal amplification methods that use enzymes or nanoparticles, radical polymerization has been explored as an approach to sensitive biodetection because of the inherent amplification in the chain-growth process. Polymerization-based biodetection benefits from different types of initiation reactions and a wide variety of monomer choices, making it adaptable to diverse sensing conditions and detection methods. This review presents the many radical polymerization chemistries that have been implemented in biodetection platforms and evaluates their utility. First, we describe the principle of each polymerization-based biodetection and discuss its advantages and current limitations for practical use in the field. Then, we compare all of the methods in terms of performance, equipment-dependence, user-friendliness, and amplification time. Finally, we highlight exciting future directions and opportunities for developing practical biosensors that use radical polymerization reactions to generate signals. |
| first_indexed | 2024-09-23T11:18:02Z |
| format | Article |
| id | mit-1721.1/123795 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:18:02Z |
| publishDate | 2020 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1237952022-09-27T18:33:17Z Radical polymerization reactions for amplified biodetection signals Kim, Seunghyeon Sikes Johnson, Hadley Massachusetts Institute of Technology. Department of Chemical Engineering Chemical reactions that provide amplified biodetection signals are essential in point-of-care diagnostics, a category of portable biosensors that should detect nanomolar to attomolar concentrations of clinically actionable biomarkers in bodily fluids without using advanced lab equipment. As an alternative to common signal amplification methods that use enzymes or nanoparticles, radical polymerization has been explored as an approach to sensitive biodetection because of the inherent amplification in the chain-growth process. Polymerization-based biodetection benefits from different types of initiation reactions and a wide variety of monomer choices, making it adaptable to diverse sensing conditions and detection methods. This review presents the many radical polymerization chemistries that have been implemented in biodetection platforms and evaluates their utility. First, we describe the principle of each polymerization-based biodetection and discuss its advantages and current limitations for practical use in the field. Then, we compare all of the methods in terms of performance, equipment-dependence, user-friendliness, and amplification time. Finally, we highlight exciting future directions and opportunities for developing practical biosensors that use radical polymerization reactions to generate signals. 2020-02-12T16:33:17Z 2020-02-12T16:33:17Z 2020-02 2019-11 Article http://purl.org/eprint/type/JournalArticle 1759-9954 1759-9962 https://hdl.handle.net/1721.1/123795 Kim, Seunghyeon and Hadley D. Sikes. "Radical polymerization reactions for amplified biodetection signals." Polymer Chemistry (February 2020): c9py01801h © 2020 Royal Society of Chemistry http://dx.doi.org/10.1039/c9py01801h Polymer Chemistry Creative Commons Attribution Noncommercial 3.0 unported license https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry (RSC) Royal Society of Chemistry (RSC) |
| spellingShingle | Kim, Seunghyeon Sikes Johnson, Hadley Radical polymerization reactions for amplified biodetection signals |
| title | Radical polymerization reactions for amplified biodetection signals |
| title_full | Radical polymerization reactions for amplified biodetection signals |
| title_fullStr | Radical polymerization reactions for amplified biodetection signals |
| title_full_unstemmed | Radical polymerization reactions for amplified biodetection signals |
| title_short | Radical polymerization reactions for amplified biodetection signals |
| title_sort | radical polymerization reactions for amplified biodetection signals |
| url | https://hdl.handle.net/1721.1/123795 |
| work_keys_str_mv | AT kimseunghyeon radicalpolymerizationreactionsforamplifiedbiodetectionsignals AT sikesjohnsonhadley radicalpolymerizationreactionsforamplifiedbiodetectionsignals |