Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels
Biominerals have been widely studied due to their unique mechanical properties, afforded by their inorganic-organic composite structure and well-controlled growth in macromolecular environments. However, a lack of suitable characterization techniques for inorganic minerals in organic-rich media has...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2020
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| Online Access: | https://hdl.handle.net/1721.1/124211 |
| _version_ | 1826201354674110464 |
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| author | Regitsky, Abigail U. (Abigail Utami) Keshavarz, Bavand McKinley, Gareth H Holten-Andersen, Niels |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Regitsky, Abigail U. (Abigail Utami) Keshavarz, Bavand McKinley, Gareth H Holten-Andersen, Niels |
| author_sort | Regitsky, Abigail U. (Abigail Utami) |
| collection | MIT |
| description | Biominerals have been widely studied due to their unique mechanical properties, afforded by their inorganic-organic composite structure and well-controlled growth in macromolecular environments. However, a lack of suitable characterization techniques for inorganic minerals in organic-rich media has prevented a full understanding of biomineralization. Here, we applied rheometry to study mineral nucleation and growth dynamics by measuring viscoelastic material properties of a hydrogel system during mineralization. Our proof-of-concept system consists of a gelatin hydrogel matrix preloaded with calcium ions and a reservoir of carbonate ions, which diffuse through the gel to initiate mineralization. We found that gels with diffused carbonate show an increase in low frequency energy dissipation, which scales with carbonate concentration and gel pH. Using this signal, and recognizing that mineralization occurs simultaneously with carbonate diffusion in our system, we have mechanoscopically tracked mineral growth in situ, showcasing the potential of rheometry for studying mineralization kinetics in real time. |
| first_indexed | 2024-09-23T11:50:36Z |
| format | Article |
| id | mit-1721.1/124211 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:50:36Z |
| publishDate | 2020 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1242112022-10-01T06:24:30Z Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels Regitsky, Abigail U. (Abigail Utami) Keshavarz, Bavand McKinley, Gareth H Holten-Andersen, Niels Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Biominerals have been widely studied due to their unique mechanical properties, afforded by their inorganic-organic composite structure and well-controlled growth in macromolecular environments. However, a lack of suitable characterization techniques for inorganic minerals in organic-rich media has prevented a full understanding of biomineralization. Here, we applied rheometry to study mineral nucleation and growth dynamics by measuring viscoelastic material properties of a hydrogel system during mineralization. Our proof-of-concept system consists of a gelatin hydrogel matrix preloaded with calcium ions and a reservoir of carbonate ions, which diffuse through the gel to initiate mineralization. We found that gels with diffused carbonate show an increase in low frequency energy dissipation, which scales with carbonate concentration and gel pH. Using this signal, and recognizing that mineralization occurs simultaneously with carbonate diffusion in our system, we have mechanoscopically tracked mineral growth in situ, showcasing the potential of rheometry for studying mineralization kinetics in real time. United States. Office of Naval Research (Grant N00014-15-1-2763) 2020-03-23T20:52:48Z 2020-03-23T20:52:48Z 2017-11 2017-10 2020-03-19T16:44:00Z Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 https://hdl.handle.net/1721.1/124211 Regitsky, Abigail U. et al. "Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels." Biomacromolecules 18, 12 (November 2017): 4067-4074 © 2017 American Chemical Society en http://dx.doi.org/10.1021/acs.biomac.7b01129 Biomacromolecules Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) Gareth McKinley via Elizabeth Soergel |
| spellingShingle | Regitsky, Abigail U. (Abigail Utami) Keshavarz, Bavand McKinley, Gareth H Holten-Andersen, Niels Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels |
| title | Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels |
| title_full | Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels |
| title_fullStr | Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels |
| title_full_unstemmed | Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels |
| title_short | Rheology as a Mechanoscopic Method to Monitor Mineralization in Hydrogels |
| title_sort | rheology as a mechanoscopic method to monitor mineralization in hydrogels |
| url | https://hdl.handle.net/1721.1/124211 |
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