A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1
Hyperglycemia fluctuation is associated with diabetes mellitus (DM) complications when compared to persistent hyperglycemia. Previous studies have shown that paeoniflorin (PF), through its antiapoptosis, anti-inflammation, and antithrombotic properties, effectively protects against cardiovascular an...
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| Format: | Article |
| Language: | English |
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Hindawi
2020
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| Online Access: | https://hdl.handle.net/1721.1/124547 |
| _version_ | 1811069073109286912 |
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| author | Wang, Jing-Shang Huang, Ye Zhang, Shuping Yin, Hui-Jun Zhang, Lei Zhang, Yan-Hong Song, Ye-Wen Li, Dan-Dan |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Wang, Jing-Shang Huang, Ye Zhang, Shuping Yin, Hui-Jun Zhang, Lei Zhang, Yan-Hong Song, Ye-Wen Li, Dan-Dan |
| author_sort | Wang, Jing-Shang |
| collection | MIT |
| description | Hyperglycemia fluctuation is associated with diabetes mellitus (DM) complications when compared to persistent hyperglycemia. Previous studies have shown that paeoniflorin (PF), through its antiapoptosis, anti-inflammation, and antithrombotic properties, effectively protects against cardiovascular and cerebrovascular disease. However, the mechanism underlying the protection from PF against vascular injuries induced by hyperglycemia fluctuations remains poorly understood. Herein, we investigated the potential protective role of PF on human umbilical vein endothelial cells (HUVECs) subjected to intermittent glucose levels in vitro and in DM rats with fluctuating hyperglycemia in vivo. A remarkable increased apoptosis associated with elevated inflammation, increased oxidative stress, and high protein level of PKCβ1 was induced in HUVECs by intermittently changing glucose for 8 days, and PF recovered those detrimental changes. LY333531, a potent PKCβ1 inhibitor, and metformin manifested similar effects. Additionally, in DM rats with fluctuating hyperglycemia, PF protected against vascular damage as what has been observed in vitro. Taken together, PF attenuates the vascular injury induced by fluctuant hyperglycemia through oxidative stress inhibition, inflammatory reaction reduction, and PKCβ1 protein level repression, suggesting its perspective clinical usage. ©2019 |
| first_indexed | 2024-09-23T08:05:16Z |
| format | Article |
| id | mit-1721.1/124547 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:05:16Z |
| publishDate | 2020 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | mit-1721.1/1245472022-09-30T07:25:37Z A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 Wang, Jing-Shang Huang, Ye Zhang, Shuping Yin, Hui-Jun Zhang, Lei Zhang, Yan-Hong Song, Ye-Wen Li, Dan-Dan Massachusetts Institute of Technology. Institute for Medical Engineering & Science Hyperglycemia fluctuation is associated with diabetes mellitus (DM) complications when compared to persistent hyperglycemia. Previous studies have shown that paeoniflorin (PF), through its antiapoptosis, anti-inflammation, and antithrombotic properties, effectively protects against cardiovascular and cerebrovascular disease. However, the mechanism underlying the protection from PF against vascular injuries induced by hyperglycemia fluctuations remains poorly understood. Herein, we investigated the potential protective role of PF on human umbilical vein endothelial cells (HUVECs) subjected to intermittent glucose levels in vitro and in DM rats with fluctuating hyperglycemia in vivo. A remarkable increased apoptosis associated with elevated inflammation, increased oxidative stress, and high protein level of PKCβ1 was induced in HUVECs by intermittently changing glucose for 8 days, and PF recovered those detrimental changes. LY333531, a potent PKCβ1 inhibitor, and metformin manifested similar effects. Additionally, in DM rats with fluctuating hyperglycemia, PF protected against vascular damage as what has been observed in vitro. Taken together, PF attenuates the vascular injury induced by fluctuant hyperglycemia through oxidative stress inhibition, inflammatory reaction reduction, and PKCβ1 protein level repression, suggesting its perspective clinical usage. ©2019 National Natural Science Foundation of China (no. 81573803) National Natural Science Foundation of China (no. 81703862) 2020-04-08T18:00:05Z 2020-04-08T18:00:05Z 2019-04 2018-10 2020-03-01T06:40:23Z Article http://purl.org/eprint/type/JournalArticle 1942-0994 1942-0900 https://hdl.handle.net/1721.1/124547 Wang, Jing-Shang, et al., "A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1." Oxidative medicine and cellular longevity 2019: no. 5647219 doi 10.1155/2019/5647219 ©2019 Author(s) en http://dx.doi.org/10.1155/2019/5647219 Oxidative medicine and cellular longevity Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Copyright © 2019 Jing-Shang Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. text/xml application/pdf Hindawi Hindawi |
| spellingShingle | Wang, Jing-Shang Huang, Ye Zhang, Shuping Yin, Hui-Jun Zhang, Lei Zhang, Yan-Hong Song, Ye-Wen Li, Dan-Dan A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 |
| title | A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 |
| title_full | A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 |
| title_fullStr | A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 |
| title_full_unstemmed | A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 |
| title_short | A protective role of paeoniflorin in fluctuant hyperglycemia-induced vascular endothelial injuries through antioxidative and anti-inflammatory effects and reduction of PKCβ1 |
| title_sort | protective role of paeoniflorin in fluctuant hyperglycemia induced vascular endothelial injuries through antioxidative and anti inflammatory effects and reduction of pkcβ1 |
| url | https://hdl.handle.net/1721.1/124547 |
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