Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells
Construction of small and continuous capillary networks is a fundamental challenge for the development of three-dimensional (3D) tissue engineering. In particular, to construct mature and stable capillary networks, it is important to consider interactions between endothelial cells and pericytes. Thi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Mary Ann Liebert Inc
2020
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| Online Access: | https://hdl.handle.net/1721.1/124556 |
| _version_ | 1826211035558707200 |
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| author | Yamamoto, Kyoko Tanimura, Kohei Watanabe, Masafumi Sano, Hiromu Uwamori, Hiroyuki Mabuchi, Yo Matsuzaki, Yumi Chung, Seok Kamm, Roger Dale Tanishita, Kazuo Sudo, Ryo |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Yamamoto, Kyoko Tanimura, Kohei Watanabe, Masafumi Sano, Hiromu Uwamori, Hiroyuki Mabuchi, Yo Matsuzaki, Yumi Chung, Seok Kamm, Roger Dale Tanishita, Kazuo Sudo, Ryo |
| author_sort | Yamamoto, Kyoko |
| collection | MIT |
| description | Construction of small and continuous capillary networks is a fundamental challenge for the development of three-dimensional (3D) tissue engineering. In particular, to construct mature and stable capillary networks, it is important to consider interactions between endothelial cells and pericytes. This study aimed to construct stable capillary networks covered by pericyte-like perivascular cells, which maintain the lumen of small diameter similar to that of capillary structures in vivo. Vascular sprouting, capillary extension, and stabilization were investigated using a 3D angiogenesis model containing human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) in a microfluidic device. A series of HUVEC:MSC ratios was tested; the ratio was found to be an important factor in the construction of capillary structures. We found that stable capillary networks that were covered by MSC-derived perivascular cells can be constructed at 1:1 HUVEC:MSC ratio. The constructed capillary networks had continuous lumens with <10-μm diameter, which were maintained for at least 21 days. This angiogenic process and basement membrane formation were regulated by HUVEC-MSC interactions. Construction of capillary networks is a fundamental challenge for the development of three-dimensional (3D) tissue engineering. However, it is not well understood how to construct stable capillary networks that maintain a luminal size similar to that of capillary structures in vivo (i.e., <10 μm diameter). In this study, we demonstrated the construction of stable capillary networks covered by pericyte-like perivascular cells using an in vitro 3D angiogenesis model by optimizing interactions between endothelial cells and perivascular cells. Our 3D angiogenesis model can be combined with 3D culture of epithelial cells in the context of vascularization of 3D tissue-engineered constructs. Keywords:3D angiogenesis model; microfluidic device; pericytes; mesenchymal stem cells |
| first_indexed | 2024-09-23T14:59:32Z |
| format | Article |
| id | mit-1721.1/124556 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:59:32Z |
| publishDate | 2020 |
| publisher | Mary Ann Liebert Inc |
| record_format | dspace |
| spelling | mit-1721.1/1245562022-10-01T23:48:27Z Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells Yamamoto, Kyoko Tanimura, Kohei Watanabe, Masafumi Sano, Hiromu Uwamori, Hiroyuki Mabuchi, Yo Matsuzaki, Yumi Chung, Seok Kamm, Roger Dale Tanishita, Kazuo Sudo, Ryo Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Construction of small and continuous capillary networks is a fundamental challenge for the development of three-dimensional (3D) tissue engineering. In particular, to construct mature and stable capillary networks, it is important to consider interactions between endothelial cells and pericytes. This study aimed to construct stable capillary networks covered by pericyte-like perivascular cells, which maintain the lumen of small diameter similar to that of capillary structures in vivo. Vascular sprouting, capillary extension, and stabilization were investigated using a 3D angiogenesis model containing human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) in a microfluidic device. A series of HUVEC:MSC ratios was tested; the ratio was found to be an important factor in the construction of capillary structures. We found that stable capillary networks that were covered by MSC-derived perivascular cells can be constructed at 1:1 HUVEC:MSC ratio. The constructed capillary networks had continuous lumens with <10-μm diameter, which were maintained for at least 21 days. This angiogenic process and basement membrane formation were regulated by HUVEC-MSC interactions. Construction of capillary networks is a fundamental challenge for the development of three-dimensional (3D) tissue engineering. However, it is not well understood how to construct stable capillary networks that maintain a luminal size similar to that of capillary structures in vivo (i.e., <10 μm diameter). In this study, we demonstrated the construction of stable capillary networks covered by pericyte-like perivascular cells using an in vitro 3D angiogenesis model by optimizing interactions between endothelial cells and perivascular cells. Our 3D angiogenesis model can be combined with 3D culture of epithelial cells in the context of vascularization of 3D tissue-engineered constructs. Keywords:3D angiogenesis model; microfluidic device; pericytes; mesenchymal stem cells Japan Society for Promotion of Science (16H03173 and 25560208) 2020-04-09T14:45:51Z 2020-04-09T14:45:51Z 2018-10 2018-07 2019-09-17T13:25:38Z Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X https://hdl.handle.net/1721.1/124556 Yamamoto, Kyoko et al. "Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells." Tissue Engineering Part A 25, 5-6 (March 2019): 499-510 ©2019, Mary Ann Liebert, Inc. en http://dx.doi.org/10.1089/ten.tea.2018.0186 Tissue Engineering Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert Inc Mary Ann Liebert |
| spellingShingle | Yamamoto, Kyoko Tanimura, Kohei Watanabe, Masafumi Sano, Hiromu Uwamori, Hiroyuki Mabuchi, Yo Matsuzaki, Yumi Chung, Seok Kamm, Roger Dale Tanishita, Kazuo Sudo, Ryo Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells |
| title | Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells |
| title_full | Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells |
| title_fullStr | Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells |
| title_full_unstemmed | Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells |
| title_short | Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells |
| title_sort | construction of continuous capillary networks stabilized by pericyte like perivascular cells |
| url | https://hdl.handle.net/1721.1/124556 |
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