Designing yeast as plant-like hyperaccumulators for heavy metals
Hyperaccumulators typically refer to plants that absorb and tolerate elevated amounts of heavy metals. Due to their unique metal trafficking abilities, hyperaccumulators are promising candidates for bioremediation applications. However, compared to bacteria-based bioremediation systems, plant life c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2020
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| Online Access: | https://hdl.handle.net/1721.1/124616 |
| _version_ | 1826197180429369344 |
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| author | Sun, George L. Reynolds, Erin. E. Belcher, Angela M. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Sun, George L. Reynolds, Erin. E. Belcher, Angela M. |
| author_sort | Sun, George L. |
| collection | MIT |
| description | Hyperaccumulators typically refer to plants that absorb and tolerate elevated amounts of heavy metals. Due to their unique metal trafficking abilities, hyperaccumulators are promising candidates for bioremediation applications. However, compared to bacteria-based bioremediation systems, plant life cycle is long and growing conditions are difficult to maintain hindering their adoption. Herein, we combine the robust growth and engineerability of bacteria with the unique waste management mechanisms of plants by using a more tractable platform-the common baker’s yeast-to create plant-like hyperaccumulators. Through overexpression of metal transporters and engineering metal trafficking pathways, engineered yeast strains are able to sequester metals at concentrations 10–100 times more than established hyperaccumulator thresholds for chromium, arsenic, and cadmium. Strains are further engineered to be selective for either cadmium or strontium removal, specifically for radioactive Sr90. Overall, this work presents a systematic approach for transforming yeast into metal hyperaccumulators that are as effective as their plant counterparts. |
| first_indexed | 2024-09-23T10:44:02Z |
| format | Article |
| id | mit-1721.1/124616 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:44:02Z |
| publishDate | 2020 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1246162022-09-27T14:35:51Z Designing yeast as plant-like hyperaccumulators for heavy metals Sun, George L. Reynolds, Erin. E. Belcher, Angela M. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Koch Institute for Integrative Cancer Research at MIT General Biochemistry, Genetics and Molecular Biology General Physics and Astronomy General Chemistry Hyperaccumulators typically refer to plants that absorb and tolerate elevated amounts of heavy metals. Due to their unique metal trafficking abilities, hyperaccumulators are promising candidates for bioremediation applications. However, compared to bacteria-based bioremediation systems, plant life cycle is long and growing conditions are difficult to maintain hindering their adoption. Herein, we combine the robust growth and engineerability of bacteria with the unique waste management mechanisms of plants by using a more tractable platform-the common baker’s yeast-to create plant-like hyperaccumulators. Through overexpression of metal transporters and engineering metal trafficking pathways, engineered yeast strains are able to sequester metals at concentrations 10–100 times more than established hyperaccumulator thresholds for chromium, arsenic, and cadmium. Strains are further engineered to be selective for either cadmium or strontium removal, specifically for radioactive Sr90. Overall, this work presents a systematic approach for transforming yeast into metal hyperaccumulators that are as effective as their plant counterparts. National Institute of Environmental Health Sciences (Grant P30-ES002109) 2020-04-14T14:20:35Z 2020-04-14T14:20:35Z 2019-11-08 2020-02-06T13:45:52Z Article http://purl.org/eprint/type/JournalArticle 2041-1723 https://hdl.handle.net/1721.1/124616 Sun, George L., Erin.E. Reynolds and Angela M. Belcher. "Designing yeast as plant-like hyperaccumulators for heavy metals." Nature Communications 10 (2019): 5080 © 2019 The Author(s) en 10.1038/s41467-019-13093-6 Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | General Biochemistry, Genetics and Molecular Biology General Physics and Astronomy General Chemistry Sun, George L. Reynolds, Erin. E. Belcher, Angela M. Designing yeast as plant-like hyperaccumulators for heavy metals |
| title | Designing yeast as plant-like hyperaccumulators for heavy metals |
| title_full | Designing yeast as plant-like hyperaccumulators for heavy metals |
| title_fullStr | Designing yeast as plant-like hyperaccumulators for heavy metals |
| title_full_unstemmed | Designing yeast as plant-like hyperaccumulators for heavy metals |
| title_short | Designing yeast as plant-like hyperaccumulators for heavy metals |
| title_sort | designing yeast as plant like hyperaccumulators for heavy metals |
| topic | General Biochemistry, Genetics and Molecular Biology General Physics and Astronomy General Chemistry |
| url | https://hdl.handle.net/1721.1/124616 |
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