Expansion microscopy: enabling single cell analysis in intact biological systems
There is a need for single cell analysis methods that enable the identification and localization of different kinds of biomolecules throughout cells and intact tissues, thereby allowing characterization and classification of individual cells and their relationships to each other within intact system...
| Main Authors: | , , |
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| 格式: | 文件 |
| 语言: | English |
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Wiley
2020
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| 在线阅读: | https://hdl.handle.net/1721.1/124633 |
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| author | Alon, Shahar Huynh, Grace H. Boyden, Edward S. |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Alon, Shahar Huynh, Grace H. Boyden, Edward S. |
| author_sort | Alon, Shahar |
| collection | MIT |
| description | There is a need for single cell analysis methods that enable the identification and localization of different kinds of biomolecules throughout cells and intact tissues, thereby allowing characterization and classification of individual cells and their relationships to each other within intact systems. Expansion microscopy (ExM) is a technology that physically magnifies tissues in an isotropic way, thereby achieving super-resolution microscopy on diffraction-limited microscopes, enabling rapid image acquisition and large field of view. As a result, ExM is well-positioned to integrate molecular content and cellular morphology, with the spatial precision sufficient to resolve individual biological building blocks, and the scale and accessibility required to deploy over extended 3-D objects like tissues and organs. ©2019 |
| first_indexed | 2024-09-23T10:16:43Z |
| format | Article |
| id | mit-1721.1/124633 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:16:43Z |
| publishDate | 2020 |
| publisher | Wiley |
| record_format | dspace |
| spelling | mit-1721.1/1246332022-09-30T20:05:45Z Expansion microscopy: enabling single cell analysis in intact biological systems Alon, Shahar Huynh, Grace H. Boyden, Edward S. Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT There is a need for single cell analysis methods that enable the identification and localization of different kinds of biomolecules throughout cells and intact tissues, thereby allowing characterization and classification of individual cells and their relationships to each other within intact systems. Expansion microscopy (ExM) is a technology that physically magnifies tissues in an isotropic way, thereby achieving super-resolution microscopy on diffraction-limited microscopes, enabling rapid image acquisition and large field of view. As a result, ExM is well-positioned to integrate molecular content and cellular morphology, with the spatial precision sufficient to resolve individual biological building blocks, and the scale and accessibility required to deploy over extended 3-D objects like tissues and organs. ©2019 IARPA (grant no. D16PC00008) NIH (grant no. 1R01MH103910) NIH (grant no. 1RM1HG008525) NIH (grant no. 1R01MH110932) NIH (grant no. 1R01EB024261) NIH (grant no. 1R01NS102727) U.S. Army Research Office (grant no. W911NF1510548) 2020-04-14T19:49:45Z 2020-04-14T19:49:45Z 2019-04 2018-06 2020-04-06T16:58:30Z Article http://purl.org/eprint/type/JournalArticle 1742-4658 https://hdl.handle.net/1721.1/124633 Alon, Shahar, Grace H. Huynh, and Edward S. Boyden, "Expansion microscopy: enabling single cell analysis in intact biological systems." FEBS journal 286, 8 (April 2019): p. 1482-94 doi 10.1111/febs.14597 2019 ©2019 Author(s) en 10.1111/febs.14597 FEBS journal Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley PMC |
| spellingShingle | Alon, Shahar Huynh, Grace H. Boyden, Edward S. Expansion microscopy: enabling single cell analysis in intact biological systems |
| title | Expansion microscopy: enabling single cell analysis in intact biological systems |
| title_full | Expansion microscopy: enabling single cell analysis in intact biological systems |
| title_fullStr | Expansion microscopy: enabling single cell analysis in intact biological systems |
| title_full_unstemmed | Expansion microscopy: enabling single cell analysis in intact biological systems |
| title_short | Expansion microscopy: enabling single cell analysis in intact biological systems |
| title_sort | expansion microscopy enabling single cell analysis in intact biological systems |
| url | https://hdl.handle.net/1721.1/124633 |
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