Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis
In many bacteria, ribosomal proteins autogenously repress their own expression by interacting with RNA structures typically located in the 5′′-UTRs of their mRNA transcripts. This regulation is necessary to maintain a balance between ribosomal proteins and rRNA to ensure proper ribosome production....
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| Format: | Article |
| Language: | English |
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Cold Spring Harbor Laboratory
2020
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| Online Access: | https://hdl.handle.net/1721.1/125170 |
| _version_ | 1826202634440146944 |
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| author | Parker, Darren J. Li, Gene-Wei |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Parker, Darren J. Li, Gene-Wei |
| author_sort | Parker, Darren J. |
| collection | MIT |
| description | In many bacteria, ribosomal proteins autogenously repress their own expression by interacting with RNA structures typically located in the 5′′-UTRs of their mRNA transcripts. This regulation is necessary to maintain a balance between ribosomal proteins and rRNA to ensure proper ribosome production. Despite advances in noncoding RNA discovery and validation of RNA-protein regulatory interactions, the selective pressures that govern the formation and maintenance of such RNA cis-regulators in the context of an organism remain largely undetermined. To examine the impact disruptions to this regulation have on bacterial fitness, we introduced point mutations that abolish ribosomal protein binding and regulation into the RNA structure that controls expression of ribosomal proteins L20 and L35 within the Bacillus subtilis genome. Our studies indicate that removing this regulation results in reduced log phase growth, improper rRNA maturation, and the accumulation of a kinetically trapped or misassembled ribosomal particle at low temperatures, suggesting defects in ribosome synthesis. Such work emphasizes the important role regulatory RNAs play in the stoichiometric production of ribosomal components for proper ribosome composition and overall organism viability and reinforces the potential of targeting ribosomal protein production and ribosome assembly with novel antimicrobials. |
| first_indexed | 2024-09-23T12:12:07Z |
| format | Article |
| id | mit-1721.1/125170 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:12:07Z |
| publishDate | 2020 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | dspace |
| spelling | mit-1721.1/1251702022-09-28T00:43:40Z Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis Parker, Darren J. Li, Gene-Wei Massachusetts Institute of Technology. Department of Biology In many bacteria, ribosomal proteins autogenously repress their own expression by interacting with RNA structures typically located in the 5′′-UTRs of their mRNA transcripts. This regulation is necessary to maintain a balance between ribosomal proteins and rRNA to ensure proper ribosome production. Despite advances in noncoding RNA discovery and validation of RNA-protein regulatory interactions, the selective pressures that govern the formation and maintenance of such RNA cis-regulators in the context of an organism remain largely undetermined. To examine the impact disruptions to this regulation have on bacterial fitness, we introduced point mutations that abolish ribosomal protein binding and regulation into the RNA structure that controls expression of ribosomal proteins L20 and L35 within the Bacillus subtilis genome. Our studies indicate that removing this regulation results in reduced log phase growth, improper rRNA maturation, and the accumulation of a kinetically trapped or misassembled ribosomal particle at low temperatures, suggesting defects in ribosome synthesis. Such work emphasizes the important role regulatory RNAs play in the stoichiometric production of ribosomal components for proper ribosome composition and overall organism viability and reinforces the potential of targeting ribosomal protein production and ribosome assembly with novel antimicrobials. National Institutes of Health (U.S.) (Grant T32GM007287) 2020-05-12T13:34:59Z 2020-05-12T13:34:59Z 2018-09 2020-01-23T17:33:10Z Article http://purl.org/eprint/type/JournalArticle 1355-8382 https://hdl.handle.net/1721.1/125170 Babina, Arrianne M. et al. “Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis.” RNA (New York, N.Y.) 24 (2018): 1133-1143 © 2018 The Author(s) en 10.1261/RNA.065011.117 RNA (New York, N.Y.) Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Cold Spring Harbor Laboratory Cold Spring Harbor Laboratory Press |
| spellingShingle | Parker, Darren J. Li, Gene-Wei Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis |
| title | Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis |
| title_full | Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis |
| title_fullStr | Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis |
| title_full_unstemmed | Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis |
| title_short | Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis |
| title_sort | fitness advantages conferred by the l20 interacting rna cis regulator of ribosomal protein synthesis in bacillus subtilis |
| url | https://hdl.handle.net/1721.1/125170 |
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