7-Deazaguanine modifications protect phage DNA from host restriction systems
Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G+), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2020
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| Online Access: | https://hdl.handle.net/1721.1/125185 |
| _version_ | 1826195121150885888 |
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| author | Hillebrand, Roman Sassanfar, Mandana Dedon, Peter C. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hillebrand, Roman Sassanfar, Mandana Dedon, Peter C. |
| author_sort | Hillebrand, Roman |
| collection | MIT |
| description | Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G+), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2′-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2′-deoxy-7-amido-7-deazaguanine (dADG), 2′-deoxy-7-cyano-7-deazaguanine (dPreQ0) and 2′-deoxy-7- aminomethyl-7-deazaguanine (dPreQ1). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2′-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes. |
| first_indexed | 2024-09-23T10:07:47Z |
| format | Article |
| id | mit-1721.1/125185 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:07:47Z |
| publishDate | 2020 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1251852022-09-30T19:03:47Z 7-Deazaguanine modifications protect phage DNA from host restriction systems Hillebrand, Roman Sassanfar, Mandana Dedon, Peter C. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Center for Environmental Health Sciences Massachusetts Institute of Technology. Department of Biology Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G+), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2′-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2′-deoxy-7-amido-7-deazaguanine (dADG), 2′-deoxy-7-cyano-7-deazaguanine (dPreQ0) and 2′-deoxy-7- aminomethyl-7-deazaguanine (dPreQ1). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2′-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes. National Institutes of Health (U.S.) (Grant GM70641) 2020-05-12T17:42:33Z 2020-05-12T17:42:33Z 2019-11 2020-01-23T13:39:05Z Article http://purl.org/eprint/type/JournalArticle 2041-1723 https://hdl.handle.net/1721.1/125185 Hutinet, Geoffrey et al. “7-Deazaguanine modifications protect phage DNA from host restriction systems.” Nature Communications 10 (2019): 5442 © 2019 The Author(s) en 10.1038/s41467-019-13384-y Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Hillebrand, Roman Sassanfar, Mandana Dedon, Peter C. 7-Deazaguanine modifications protect phage DNA from host restriction systems |
| title | 7-Deazaguanine modifications protect phage DNA from host restriction systems |
| title_full | 7-Deazaguanine modifications protect phage DNA from host restriction systems |
| title_fullStr | 7-Deazaguanine modifications protect phage DNA from host restriction systems |
| title_full_unstemmed | 7-Deazaguanine modifications protect phage DNA from host restriction systems |
| title_short | 7-Deazaguanine modifications protect phage DNA from host restriction systems |
| title_sort | 7 deazaguanine modifications protect phage dna from host restriction systems |
| url | https://hdl.handle.net/1721.1/125185 |
| work_keys_str_mv | AT hillebrandroman 7deazaguaninemodificationsprotectphagednafromhostrestrictionsystems AT sassanfarmandana 7deazaguaninemodificationsprotectphagednafromhostrestrictionsystems AT dedonpeterc 7deazaguaninemodificationsprotectphagednafromhostrestrictionsystems |