Efficient 15N–13C Polarization Transfer by Third-Spin-Assisted Pulsed Cross-Polarization Magic-Angle-Spinning NMR for Protein Structure Determination

Efficient 15N–13C Polarization Transfer by Third-Spin-Assisted Pulsed Cross-Polarization Magic-Angle-Spinning NMR for Protein Structure Determination

We introduce a pulsed third-spin-assisted recoupling experiment that produces high-intensity long-range 15N-13C cross peaks using low radiofrequency (rf) energy. This Proton-Enhanced Rotor-echo Short-Pulse IRradiATION Cross-Polarization (PERSPIRATIONCP) pulse sequence operates with the same principl...

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Bibliographic Details
Main Authors:	Gelenter, Martin David, Hong, Mei
Other Authors:	Massachusetts Institute of Technology. Department of Chemistry
Format:	Article
Language:	English
Published:	American Chemical Society (ACS) 2020
Online Access:	https://hdl.handle.net/1721.1/125215

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