Excised linear introns regulate growth in yeast
Spliceosomal introns are ubiquitous non-coding RNAs that are typically destined for rapid debranching and degradation. Here we describe 34 excised introns in Saccharomyces cerevisiae that—despite being rapidly degraded in log-phase growth—accumulate as linear RNAs under either saturated-growth condi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2020
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| Online Access: | https://hdl.handle.net/1721.1/125253 |
| _version_ | 1826213566221385728 |
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| author | Morgan, Jeffrey T. (Jeffrey Thomas) Bartel, David Fink, Gerald R |
| author2 | Whitehead Institute for Biomedical Research |
| author_facet | Whitehead Institute for Biomedical Research Morgan, Jeffrey T. (Jeffrey Thomas) Bartel, David Fink, Gerald R |
| author_sort | Morgan, Jeffrey T. (Jeffrey Thomas) |
| collection | MIT |
| description | Spliceosomal introns are ubiquitous non-coding RNAs that are typically destined for rapid debranching and degradation. Here we describe 34 excised introns in Saccharomyces cerevisiae that—despite being rapidly degraded in log-phase growth—accumulate as linear RNAs under either saturated-growth conditions or other stresses that cause prolonged inhibition of TORC1, which is a key integrator of growth signalling. Introns that become stabilized remain associated with components of the spliceosome and differ from other spliceosomal introns in having a short distance between their lariat branch point and 3′ splice site, which is necessary and sufficient for their stabilization. Deletion of these unusual introns is disadvantageous in saturated conditions and causes aberrantly high growth rates in yeast that are chronically challenged with the TORC1 inhibitor rapamycin. The reintroduction of native or engineered stable introns suppresses this aberrant rapamycin response. Thus, excised introns function within the TOR growth-signalling network of S. cerevisiae and, more generally, excised spliceosomal introns can have biological functions. Keywords: Non-coding RNAs; RNA; TOR signalling; Transcriptomics |
| first_indexed | 2024-09-23T15:51:16Z |
| format | Article |
| id | mit-1721.1/125253 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:51:16Z |
| publishDate | 2020 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1252532022-10-02T04:36:10Z Excised linear introns regulate growth in yeast Morgan, Jeffrey T. (Jeffrey Thomas) Bartel, David Fink, Gerald R Whitehead Institute for Biomedical Research Massachusetts Institute of Technology. Department of Biology Spliceosomal introns are ubiquitous non-coding RNAs that are typically destined for rapid debranching and degradation. Here we describe 34 excised introns in Saccharomyces cerevisiae that—despite being rapidly degraded in log-phase growth—accumulate as linear RNAs under either saturated-growth conditions or other stresses that cause prolonged inhibition of TORC1, which is a key integrator of growth signalling. Introns that become stabilized remain associated with components of the spliceosome and differ from other spliceosomal introns in having a short distance between their lariat branch point and 3′ splice site, which is necessary and sufficient for their stabilization. Deletion of these unusual introns is disadvantageous in saturated conditions and causes aberrantly high growth rates in yeast that are chronically challenged with the TORC1 inhibitor rapamycin. The reintroduction of native or engineered stable introns suppresses this aberrant rapamycin response. Thus, excised introns function within the TOR growth-signalling network of S. cerevisiae and, more generally, excised spliceosomal introns can have biological functions. Keywords: Non-coding RNAs; RNA; TOR signalling; Transcriptomics NIH (Grant GM035010) NIH (Grant GM118135) 2020-05-15T12:49:32Z 2020-05-15T12:49:32Z 2019-01 2017-12 2019-11-26T18:15:58Z Article http://purl.org/eprint/type/JournalArticle 0028-0836 1476-4687 https://hdl.handle.net/1721.1/125253 Morgan1, Jeffrey T., Fink, Gerald R. and Bartel, David P. "Excised linear introns regulate growth in yeast." Nature 565, 7741 (January 2019): 606–611 © 2019, Springer Nature Limited. en http://dx.doi.org/10.1038/s41586-018-0828-1 Nature Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Nature PMC |
| spellingShingle | Morgan, Jeffrey T. (Jeffrey Thomas) Bartel, David Fink, Gerald R Excised linear introns regulate growth in yeast |
| title | Excised linear introns regulate growth in yeast |
| title_full | Excised linear introns regulate growth in yeast |
| title_fullStr | Excised linear introns regulate growth in yeast |
| title_full_unstemmed | Excised linear introns regulate growth in yeast |
| title_short | Excised linear introns regulate growth in yeast |
| title_sort | excised linear introns regulate growth in yeast |
| url | https://hdl.handle.net/1721.1/125253 |
| work_keys_str_mv | AT morganjeffreytjeffreythomas excisedlinearintronsregulategrowthinyeast AT barteldavid excisedlinearintronsregulategrowthinyeast AT finkgeraldr excisedlinearintronsregulategrowthinyeast |