Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants
The precise deployment of functional payloads to plant tissues is a new approach to help advance the fundamental understanding of plant biology and accelerate plant engineering. Here, the design of a silk‐based biomaterial is reported to fabricate a microneedle‐like device, dubbed “phytoinjector,” c...
| Main Authors: | , , , , |
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| Format: | Article |
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Wiley
2020
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| Online Access: | https://hdl.handle.net/1721.1/125407 |
| _version_ | 1826204855145857024 |
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| author | Cao, Yunteng Lim, Eugene J. Xu, Menglong Weng, Jing-Ke Marelli, Benedetto |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Cao, Yunteng Lim, Eugene J. Xu, Menglong Weng, Jing-Ke Marelli, Benedetto |
| author_sort | Cao, Yunteng |
| collection | MIT |
| description | The precise deployment of functional payloads to plant tissues is a new approach to help advance the fundamental understanding of plant biology and accelerate plant engineering. Here, the design of a silk‐based biomaterial is reported to fabricate a microneedle‐like device, dubbed “phytoinjector,” capable of delivering a variety of payloads ranging from small molecules to large proteins into specific loci of various plant tissues. It is shown that phytoinjector can be used to deliver payloads into plant vasculature to study material transport in xylem and phloem and to perform complex biochemical reactions in situ. In another application, it is demonstrated Agrobacterium ‐mediated gene transfer to shoot apical meristem (SAM) and leaves at various stages of growth. Tuning of the material composition enables the fabrication of another device, dubbed “phytosampler,” which is used to precisely sample plant sap. The design of plant‐specific biomaterials to fabricate devices for drug delivery in planta opens new avenues to enhance plant resistance to biotic and abiotic stresses, provides new tools for diagnostics, and enables new opportunities in plant engineering. |
| first_indexed | 2024-09-23T13:02:09Z |
| format | Article |
| id | mit-1721.1/125407 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:02:09Z |
| publishDate | 2020 |
| publisher | Wiley |
| record_format | dspace |
| spelling | mit-1721.1/1254072022-10-01T12:42:08Z Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants Cao, Yunteng Lim, Eugene J. Xu, Menglong Weng, Jing-Ke Marelli, Benedetto Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research General Engineering General Physics and Astronomy General Materials Science Medicine (miscellaneous) General Chemical Engineering Biochemistry, Genetics and Molecular Biology (miscellaneous) The precise deployment of functional payloads to plant tissues is a new approach to help advance the fundamental understanding of plant biology and accelerate plant engineering. Here, the design of a silk‐based biomaterial is reported to fabricate a microneedle‐like device, dubbed “phytoinjector,” capable of delivering a variety of payloads ranging from small molecules to large proteins into specific loci of various plant tissues. It is shown that phytoinjector can be used to deliver payloads into plant vasculature to study material transport in xylem and phloem and to perform complex biochemical reactions in situ. In another application, it is demonstrated Agrobacterium ‐mediated gene transfer to shoot apical meristem (SAM) and leaves at various stages of growth. Tuning of the material composition enables the fabrication of another device, dubbed “phytosampler,” which is used to precisely sample plant sap. The design of plant‐specific biomaterials to fabricate devices for drug delivery in planta opens new avenues to enhance plant resistance to biotic and abiotic stresses, provides new tools for diagnostics, and enables new opportunities in plant engineering. United States. Office of Naval Research (Award No. N000141812258) National Science Foundation (U.S.) (Award No. CMMI-1752172) W. M. Keck Foundation 2020-05-22T14:11:56Z 2020-05-22T14:11:56Z 2020-04-22 2020-03 Article http://purl.org/eprint/type/JournalArticle 2198-3844 https://hdl.handle.net/1721.1/125407 Cao, Yunteng et al. "Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants." Advanced Science (April 2020): 1903551 © 2020 The Authors. https://doi.org/10.1002/advs.201903551 Advanced Science Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Wiley Wiley |
| spellingShingle | General Engineering General Physics and Astronomy General Materials Science Medicine (miscellaneous) General Chemical Engineering Biochemistry, Genetics and Molecular Biology (miscellaneous) Cao, Yunteng Lim, Eugene J. Xu, Menglong Weng, Jing-Ke Marelli, Benedetto Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants |
| title | Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants |
| title_full | Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants |
| title_fullStr | Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants |
| title_full_unstemmed | Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants |
| title_short | Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants |
| title_sort | precision delivery of multiscale payloads to tissue specific targets in plants |
| topic | General Engineering General Physics and Astronomy General Materials Science Medicine (miscellaneous) General Chemical Engineering Biochemistry, Genetics and Molecular Biology (miscellaneous) |
| url | https://hdl.handle.net/1721.1/125407 |
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