Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release
Signaling from the postsynaptic compartment regulates multiple aspects of synaptic development and function. Syntaxin 4 (Syx4) is a plasma membrane t-SNARE that promotes the growth and plasticity of Drosophila neuromuscular junctions (NMJs) by regulating the localization of key synaptic proteins in...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Informa UK Limited
2020
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| Online Access: | https://hdl.handle.net/1721.1/125444 |
| _version_ | 1826203576977850368 |
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| author | Harris, Kathryn P. Littleton, J. Troy Stewart, Bryan A. |
| author2 | Picower Institute for Learning and Memory |
| author_facet | Picower Institute for Learning and Memory Harris, Kathryn P. Littleton, J. Troy Stewart, Bryan A. |
| author_sort | Harris, Kathryn P. |
| collection | MIT |
| description | Signaling from the postsynaptic compartment regulates multiple aspects of synaptic development and function. Syntaxin 4 (Syx4) is a plasma membrane t-SNARE that promotes the growth and plasticity of Drosophila neuromuscular junctions (NMJs) by regulating the localization of key synaptic proteins in the postsynaptic compartment. Here, we describe electrophysiological analyses and report that loss of Syx4 leads to enhanced neurotransmitter release, despite a decrease in the number of active zones. We describe a requirement for postsynaptic Syx4 in regulating several presynaptic parameters, including Ca2+ cooperativity and the abundance of the presynaptic calcium channel Cacophony (Cac) at active zones. These findings indicate Syx4 negatively regulates presynaptic neurotransmitter release through a retrograde signaling mechanism from the postsynaptic compartment. |
| first_indexed | 2024-09-23T12:39:27Z |
| format | Article |
| id | mit-1721.1/125444 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:39:27Z |
| publishDate | 2020 |
| publisher | Informa UK Limited |
| record_format | dspace |
| spelling | mit-1721.1/1254442022-10-01T10:19:17Z Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release Harris, Kathryn P. Littleton, J. Troy Stewart, Bryan A. Picower Institute for Learning and Memory Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biology Signaling from the postsynaptic compartment regulates multiple aspects of synaptic development and function. Syntaxin 4 (Syx4) is a plasma membrane t-SNARE that promotes the growth and plasticity of Drosophila neuromuscular junctions (NMJs) by regulating the localization of key synaptic proteins in the postsynaptic compartment. Here, we describe electrophysiological analyses and report that loss of Syx4 leads to enhanced neurotransmitter release, despite a decrease in the number of active zones. We describe a requirement for postsynaptic Syx4 in regulating several presynaptic parameters, including Ca2+ cooperativity and the abundance of the presynaptic calcium channel Cacophony (Cac) at active zones. These findings indicate Syx4 negatively regulates presynaptic neurotransmitter release through a retrograde signaling mechanism from the postsynaptic compartment. 2020-05-26T13:56:53Z 2020-05-26T13:56:53Z 2018-09 2018-03 2020-01-24T17:54:19Z Article http://purl.org/eprint/type/JournalArticle 0167-7063 1563-5260 https://hdl.handle.net/1721.1/125444 Harris, Kathryn P. et al. "Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release." Journal of Neurogenetics 32, 3 (September 2018): 221-229 © 2018 Informa UK Limited en http://dx.doi.org/10.1080/01677063.2018.1501372 Journal of Neurogenetics Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Informa UK Limited PMC |
| spellingShingle | Harris, Kathryn P. Littleton, J. Troy Stewart, Bryan A. Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| title | Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| title_full | Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| title_fullStr | Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| title_full_unstemmed | Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| title_short | Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| title_sort | postsynaptic syntaxin 4 negatively regulates the efficiency of neurotransmitter release |
| url | https://hdl.handle.net/1721.1/125444 |
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