Mammalian cell growth dynamics in mitosis
The extent and dynamics of animal cell biomass accumulation during mitosis are unknown, primarily because growth has not been quantified with sufficient precision and temporal resolution. Using the suspended microchannel resonator and protein synthesis assays, we quantify mass accumulation and trans...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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eLife Sciences Publications, Ltd
2020
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| Online Access: | https://hdl.handle.net/1721.1/125506 |
| _version_ | 1826216119427399680 |
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| author | Miettinen, Teemu P Kang, Joon Ho Yang, Lucy F. Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Department of Physics |
| author_facet | Massachusetts Institute of Technology. Department of Physics Miettinen, Teemu P Kang, Joon Ho Yang, Lucy F. Manalis, Scott R |
| author_sort | Miettinen, Teemu P |
| collection | MIT |
| description | The extent and dynamics of animal cell biomass accumulation during mitosis are unknown, primarily because growth has not been quantified with sufficient precision and temporal resolution. Using the suspended microchannel resonator and protein synthesis assays, we quantify mass accumulation and translation rates between mitotic stages on a single-cell level. For various animal cell types, growth rates in prophase are commensurate with or higher than interphase growth rates. Growth is only stopped as cells approach metaphase-to-anaphase transition and growth resumes in late cytokinesis. Mitotic arrests stop growth independently of arresting mechanism. For mouse lymphoblast cells, growth in prophase is promoted by CDK1 through increased phosphorylation of 4E-BP1 and cap-dependent protein synthesis. Inhibition of CDK1- driven mitotic translation reduces daughter cell growth. Overall, our measurements counter the traditional dogma that growth during mitosis is negligible and provide insight into antimitotic cancer chemotherapies. |
| first_indexed | 2024-09-23T16:42:38Z |
| format | Article |
| id | mit-1721.1/125506 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:42:38Z |
| publishDate | 2020 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1255062022-09-29T20:59:29Z Mammalian cell growth dynamics in mitosis Miettinen, Teemu P Kang, Joon Ho Yang, Lucy F. Manalis, Scott R Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Koch Institute for Integrative Cancer Research at MIT The extent and dynamics of animal cell biomass accumulation during mitosis are unknown, primarily because growth has not been quantified with sufficient precision and temporal resolution. Using the suspended microchannel resonator and protein synthesis assays, we quantify mass accumulation and translation rates between mitotic stages on a single-cell level. For various animal cell types, growth rates in prophase are commensurate with or higher than interphase growth rates. Growth is only stopped as cells approach metaphase-to-anaphase transition and growth resumes in late cytokinesis. Mitotic arrests stop growth independently of arresting mechanism. For mouse lymphoblast cells, growth in prophase is promoted by CDK1 through increased phosphorylation of 4E-BP1 and cap-dependent protein synthesis. Inhibition of CDK1- driven mitotic translation reduces daughter cell growth. Overall, our measurements counter the traditional dogma that growth during mitosis is negligible and provide insight into antimitotic cancer chemotherapies. National Cancer Institute (Grant CA217377) Koch Institute (Grant P30-CA14051) 2020-05-27T16:25:22Z 2020-05-27T16:25:22Z 2019-05 2018-12 2020-01-15T18:11:16Z Article http://purl.org/eprint/type/JournalArticle 2050-084X https://hdl.handle.net/1721.1/125506 Miettinen et al. "Mammalian cell growth dynamics in mitosis." eLife 8: e44700 © 2019 The Author(s) en http://dx.doi.org/10.7554/elife.44700 eLife Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Miettinen, Teemu P Kang, Joon Ho Yang, Lucy F. Manalis, Scott R Mammalian cell growth dynamics in mitosis |
| title | Mammalian cell growth dynamics in mitosis |
| title_full | Mammalian cell growth dynamics in mitosis |
| title_fullStr | Mammalian cell growth dynamics in mitosis |
| title_full_unstemmed | Mammalian cell growth dynamics in mitosis |
| title_short | Mammalian cell growth dynamics in mitosis |
| title_sort | mammalian cell growth dynamics in mitosis |
| url | https://hdl.handle.net/1721.1/125506 |
| work_keys_str_mv | AT miettinenteemup mammaliancellgrowthdynamicsinmitosis AT kangjoonho mammaliancellgrowthdynamicsinmitosis AT yanglucyf mammaliancellgrowthdynamicsinmitosis AT manalisscottr mammaliancellgrowthdynamicsinmitosis |