Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions
Reliable early-stage detection of foodborne pathogens is a global public health challenge that requires new and improved sensing strategies. Here, we demonstrate that dynamically reconfigurable fluorescent double emulsions can function as highly responsive optical sensors for the rapid detection of...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society (ACS)
2020
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| Online Access: | https://hdl.handle.net/1721.1/125598 |
| _version_ | 1826190585598312448 |
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| author | Zeininger, Lukas Nagelberg, Sara Nicole Harvey, Kent S. Savagatrup, Suchol Herbert, Myles Benton Yoshinaga, Kosuke Kolle, Mathias Swager, Timothy M |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Zeininger, Lukas Nagelberg, Sara Nicole Harvey, Kent S. Savagatrup, Suchol Herbert, Myles Benton Yoshinaga, Kosuke Kolle, Mathias Swager, Timothy M |
| author_sort | Zeininger, Lukas |
| collection | MIT |
| description | Reliable early-stage detection of foodborne pathogens is a global public health challenge that requires new and improved sensing strategies. Here, we demonstrate that dynamically reconfigurable fluorescent double emulsions can function as highly responsive optical sensors for the rapid detection of carbohydrates fructose, glucose, mannose, and mannan, which are involved in many biological and pathogenic phenomena. The proposed detection strategy relies on reversible reactions between boronic acid surfactants and carbohydrates at the hydrocarbon/water interface leading to a dynamic reconfiguration of the droplet morphology, which alters the angular distribution of the droplet's fluorescent light emission. We exploit this unique chemical-morphological-optical coupling to detect Salmonella enterica, a type of bacteria with a well-known binding affinity for mannose. We further demonstrate an oriented immobilization of antibodies at the droplet interface to permit higher selectivity. Our demonstrations yield a new, inexpensive, robust, and generalizable sensing strategy that can help to facilitate the early detection of foodborne pathogens. |
| first_indexed | 2024-09-23T08:42:36Z |
| format | Article |
| id | mit-1721.1/125598 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:42:36Z |
| publishDate | 2020 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1255982022-09-30T10:41:13Z Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions Zeininger, Lukas Nagelberg, Sara Nicole Harvey, Kent S. Savagatrup, Suchol Herbert, Myles Benton Yoshinaga, Kosuke Kolle, Mathias Swager, Timothy M Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Mechanical Engineering Reliable early-stage detection of foodborne pathogens is a global public health challenge that requires new and improved sensing strategies. Here, we demonstrate that dynamically reconfigurable fluorescent double emulsions can function as highly responsive optical sensors for the rapid detection of carbohydrates fructose, glucose, mannose, and mannan, which are involved in many biological and pathogenic phenomena. The proposed detection strategy relies on reversible reactions between boronic acid surfactants and carbohydrates at the hydrocarbon/water interface leading to a dynamic reconfiguration of the droplet morphology, which alters the angular distribution of the droplet's fluorescent light emission. We exploit this unique chemical-morphological-optical coupling to detect Salmonella enterica, a type of bacteria with a well-known binding affinity for mannose. We further demonstrate an oriented immobilization of antibodies at the droplet interface to permit higher selectivity. Our demonstrations yield a new, inexpensive, robust, and generalizable sensing strategy that can help to facilitate the early detection of foodborne pathogens. National Institute of General Medical Sciences (U.S.) (Grant GM095843) National Science Foundation (U.S.) (Grant DMREF-1533985) German Research Foundation (Fellowship ZE1121/1-1) United States. Army Research Office (Cooperative Agreement W911NF-18-2-0048) United States. Agriculture Research Service (Agreement 8072-42000-084) 2020-06-01T16:41:56Z 2020-06-01T16:41:56Z 2019-04 2020-01-14T13:53:15Z Article http://purl.org/eprint/type/JournalArticle 2374-7951 2374-7943 https://hdl.handle.net/1721.1/125598 Zeininger, Lukas et al. “Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions.” ACS central science 5 (2019): 789-795 © 2019 The Author(s) en https://dx.doi.org/10.1021/ACSCENTSCI.9B00059 ACS central science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Zeininger, Lukas Nagelberg, Sara Nicole Harvey, Kent S. Savagatrup, Suchol Herbert, Myles Benton Yoshinaga, Kosuke Kolle, Mathias Swager, Timothy M Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions |
| title | Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions |
| title_full | Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions |
| title_fullStr | Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions |
| title_full_unstemmed | Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions |
| title_short | Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions |
| title_sort | rapid detection of salmonella enterica via directional emission from carbohydrate functionalized dynamic double emulsions |
| url | https://hdl.handle.net/1721.1/125598 |
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