Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins
Binding protein scaffolds, such as rcSso7d, have been investigated for use in diagnostic tests; however, the functional performance of rcSso7d has not yet been studied in comparison to antibodies. Here, we assessed the analyte-binding capabilities of rcSso7d and antibodies on cellulose with samples...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Royal Society of Chemistry (RSC)
2020
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| Online Access: | https://hdl.handle.net/1721.1/125627 |
| _version_ | 1826202828760154112 |
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| author | Sung, Ki-Joo Jabbour Al Maalouf, Yara Johns, Quinlan R. Miller, Eric Alexander Sikes Johnson, Hadley |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Sung, Ki-Joo Jabbour Al Maalouf, Yara Johns, Quinlan R. Miller, Eric Alexander Sikes Johnson, Hadley |
| author_sort | Sung, Ki-Joo |
| collection | MIT |
| description | Binding protein scaffolds, such as rcSso7d, have been investigated for use in diagnostic tests; however, the functional performance of rcSso7d has not yet been studied in comparison to antibodies. Here, we assessed the analyte-binding capabilities of rcSso7d and antibodies on cellulose with samples in buffer and 100% human serum. |
| first_indexed | 2024-09-23T12:20:37Z |
| format | Article |
| id | mit-1721.1/125627 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:20:37Z |
| publishDate | 2020 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1256272022-09-28T01:04:01Z Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins Sung, Ki-Joo Jabbour Al Maalouf, Yara Johns, Quinlan R. Miller, Eric Alexander Sikes Johnson, Hadley Massachusetts Institute of Technology. Department of Chemical Engineering Binding protein scaffolds, such as rcSso7d, have been investigated for use in diagnostic tests; however, the functional performance of rcSso7d has not yet been studied in comparison to antibodies. Here, we assessed the analyte-binding capabilities of rcSso7d and antibodies on cellulose with samples in buffer and 100% human serum. NSF GRFP (1122374) 2020-06-02T18:27:39Z 2020-06-02T18:27:39Z 2020-03 2020-02 2020-05-18T15:00:53Z Article http://purl.org/eprint/type/JournalArticle 0003-2654 1364-5528 https://hdl.handle.net/1721.1/125627 Sung, Ki-Joo, et al. "Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins." Analyst, 145 (2020): 2515-2519. © The Royal Society of Chemistry 2020. en http://dx.doi.org/10.1039/d0an00299b Analyst Creative Commons Attribution Noncommercial 3.0 unported license https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry (RSC) Royal Society of Chemistry (RSC) |
| spellingShingle | Sung, Ki-Joo Jabbour Al Maalouf, Yara Johns, Quinlan R. Miller, Eric Alexander Sikes Johnson, Hadley Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins |
| title | Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins |
| title_full | Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins |
| title_fullStr | Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins |
| title_full_unstemmed | Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins |
| title_short | Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins |
| title_sort | functional comparison of paper based immunoassays based on antibodies and engineered binding proteins |
| url | https://hdl.handle.net/1721.1/125627 |
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