Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device
Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society (ACS)
2020
|
| Online Access: | https://hdl.handle.net/1721.1/125688 |
| _version_ | 1811069014484451328 |
|---|---|
| author | Lee, Hyewon Lee, Jiseok Doyle, Patrick S |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lee, Hyewon Lee, Jiseok Doyle, Patrick S |
| author_sort | Lee, Hyewon |
| collection | MIT |
| description | Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling of miRNAs, without the use of complex external equipment for fluidics and imaging. For sensitive and reliable measurement without the risk of sequence bias, we employed a gold deposition-based signal amplification scheme and dark-field imaging, and seamlessly integrated a previously developed miRNA assay scheme into this platform. The assay demonstrated a limit of detection of 260 fM, along with multiplexing of small panels of miRNAs in healthy and cancer samples. We anticipate this versatile platform to facilitate a broad range of POC profiling of miRNAs in cancer-associated dysregulation with high-confidence by exploiting the unique features of hydrogel substrate in an on-chip format and colorimetric analysis. |
| first_indexed | 2024-09-23T08:04:20Z |
| format | Article |
| id | mit-1721.1/125688 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:04:20Z |
| publishDate | 2020 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1256882022-09-23T10:43:38Z Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device Lee, Hyewon Lee, Jiseok Doyle, Patrick S Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling of miRNAs, without the use of complex external equipment for fluidics and imaging. For sensitive and reliable measurement without the risk of sequence bias, we employed a gold deposition-based signal amplification scheme and dark-field imaging, and seamlessly integrated a previously developed miRNA assay scheme into this platform. The assay demonstrated a limit of detection of 260 fM, along with multiplexing of small panels of miRNAs in healthy and cancer samples. We anticipate this versatile platform to facilitate a broad range of POC profiling of miRNAs in cancer-associated dysregulation with high-confidence by exploiting the unique features of hydrogel substrate in an on-chip format and colorimetric analysis. National Institute of Biomedical Imaging and Bioengineering (Grant 5R21EB024101-02) Korea (South). Ministry of Science, ICT and Future Planning (Grant 2018M3D3A1A01055732) National Research Foundation of Korea (Grant 2018R1C1B6004086) 2020-06-05T15:08:59Z 2020-06-05T15:08:59Z 2020-03 2020-05-18T16:34:28Z Article http://purl.org/eprint/type/JournalArticle 1520-6882 https://hdl.handle.net/1721.1/125688 Lee, Hyewon et al. “Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device” Analytical Chemistry, vol. 92, no. 8, 2020, pp. 5750-5755 © 2020 The Author(s) en https://dx.doi.org/10.1021/acs.analchem.9b05043 Analytical Chemistry Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Lee, Hyewon Lee, Jiseok Doyle, Patrick S Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device |
| title | Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device |
| title_full | Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device |
| title_fullStr | Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device |
| title_full_unstemmed | Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device |
| title_short | Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device |
| title_sort | hydrogel based colorimetric assay for multiplexed microrna detection in a microfluidic device |
| url | https://hdl.handle.net/1721.1/125688 |
| work_keys_str_mv | AT leehyewon hydrogelbasedcolorimetricassayformultiplexedmicrornadetectioninamicrofluidicdevice AT leejiseok hydrogelbasedcolorimetricassayformultiplexedmicrornadetectioninamicrofluidicdevice AT doylepatricks hydrogelbasedcolorimetricassayformultiplexedmicrornadetectioninamicrofluidicdevice |