Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice
The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation i...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications, Ltd
2020
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| Online Access: | https://hdl.handle.net/1721.1/125746 |
| _version_ | 1826209187503276032 |
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| author | Kojima, Mina de Rooij, Dirk G. Page, David C |
| author2 | Whitehead Institute for Biomedical Research |
| author_facet | Whitehead Institute for Biomedical Research Kojima, Mina de Rooij, Dirk G. Page, David C |
| author_sort | Kojima, Mina |
| collection | MIT |
| description | The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation in mice, and we found that initiation involves the dramatic upregulation of a transcriptional network of thousands of genes whose expression is not limited to meiosis. This broad gene expression program is directly upregulated by STRA8, encoded by a germ cell-specific gene required for meiotic initiation. STRA8 binds its own promoter and those of thousands of other genes, including meiotic prophase genes, factors mediating DNA replication and the G1-S cell-cycle transition, and genes that promote the lengthy prophase unique to meiosis I. We conclude that, in mice, the robust amplification of this extraordinarily broad transcription program by a common factor triggers initiation of meiosis. |
| first_indexed | 2024-09-23T14:18:55Z |
| format | Article |
| id | mit-1721.1/125746 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:18:55Z |
| publishDate | 2020 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1257462022-10-01T20:33:39Z Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice Kojima, Mina de Rooij, Dirk G. Page, David C Whitehead Institute for Biomedical Research Massachusetts Institute of Technology. Department of Biology The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation in mice, and we found that initiation involves the dramatic upregulation of a transcriptional network of thousands of genes whose expression is not limited to meiosis. This broad gene expression program is directly upregulated by STRA8, encoded by a germ cell-specific gene required for meiotic initiation. STRA8 binds its own promoter and those of thousands of other genes, including meiotic prophase genes, factors mediating DNA replication and the G1-S cell-cycle transition, and genes that promote the lengthy prophase unique to meiosis I. We conclude that, in mice, the robust amplification of this extraordinarily broad transcription program by a common factor triggers initiation of meiosis. 2020-06-09T19:58:52Z 2020-06-09T19:58:52Z 2019-02 2018-11 2020-01-15T15:43:23Z Article http://purl.org/eprint/type/JournalArticle 2050-084X https://hdl.handle.net/1721.1/125746 Kojima, Mina, Dirk G. de Rooij, and David C. Page, "Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice." eLife 2019 (Feb. 2019): doi 10.7554/ELIFE.43738 ©2019 Author(s) en 10.7554/ELIFE.43738 eLife Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Kojima, Mina de Rooij, Dirk G. Page, David C Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| title | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| title_full | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| title_fullStr | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| title_full_unstemmed | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| title_short | Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| title_sort | amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice |
| url | https://hdl.handle.net/1721.1/125746 |
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