Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell
Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of tw...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2020
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| Online Access: | https://hdl.handle.net/1721.1/125780 |
| _version_ | 1826213663455838208 |
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| author | Chen, Guan-Yu Li, Zeyang Duarte, Joao N. Esteban, Alexandre Cheloha, Ross W. Theile, Christopher S Ploegh, Hidde Fink, Gerald R |
| author2 | Whitehead Institute for Biomedical Research |
| author_facet | Whitehead Institute for Biomedical Research Chen, Guan-Yu Li, Zeyang Duarte, Joao N. Esteban, Alexandre Cheloha, Ross W. Theile, Christopher S Ploegh, Hidde Fink, Gerald R |
| author_sort | Chen, Guan-Yu |
| collection | MIT |
| description | Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge. Combining the chemical and optical properties of GO with the versatility of the VHH scaffold in the context of a flow system provides a quick and efficient method for the capture and characterization of functional leukocytes. Keywords: Single domain antibody, Graphene oxide, Sortase, Enzymatic labeling, Cell detection, Leukocytes |
| first_indexed | 2024-09-23T15:52:51Z |
| format | Article |
| id | mit-1721.1/125780 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:52:51Z |
| publishDate | 2020 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1257802022-09-29T16:46:46Z Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell Chen, Guan-Yu Li, Zeyang Duarte, Joao N. Esteban, Alexandre Cheloha, Ross W. Theile, Christopher S Ploegh, Hidde Fink, Gerald R Whitehead Institute for Biomedical Research Massachusetts Institute of Technology. Department of Biology Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge. Combining the chemical and optical properties of GO with the versatility of the VHH scaffold in the context of a flow system provides a quick and efficient method for the capture and characterization of functional leukocytes. Keywords: Single domain antibody, Graphene oxide, Sortase, Enzymatic labeling, Cell detection, Leukocytes National Institutes of Health (U.S.) (NIH Grant no. 4DP1GM106409) National Institutes of Health (U.S.) (NIH Grant no. R01AI87879) Taiwan. Ministry of Science and Technology (MOST 105-2633-B-009-003) Taiwan. Ministry of Science and Technology (MOST 105-2628-B-009-001-MY3) National Chiao Tung University (Taiwan) (105W970) 2020-06-12T14:33:59Z 2020-06-12T14:33:59Z 2017-03 2016-10 2019-09-18T12:47:55Z Article http://purl.org/eprint/type/JournalArticle 0956-5663 0265-928X https://hdl.handle.net/1721.1/125780 Chen, Guan-Yu, Zeyang Li, Joao N. Duarte et al. "Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell" Biosensors and Bioelectronics 89,2 (March 2017):789-794. © 2016 Elsevier B.V. en http://dx.doi.org/10.1016/J.BIOS.2016.10.015 Biosensors and Bioelectronics Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Chen, Guan-Yu Li, Zeyang Duarte, Joao N. Esteban, Alexandre Cheloha, Ross W. Theile, Christopher S Ploegh, Hidde Fink, Gerald R Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell |
| title | Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell |
| title_full | Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell |
| title_fullStr | Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell |
| title_full_unstemmed | Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell |
| title_short | Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell |
| title_sort | rapid capture and labeling of cells on single domain antibodies functionalized flow cell |
| url | https://hdl.handle.net/1721.1/125780 |
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