Engineering of a high lipid producing Yarrowia lipolytica strain
Background: Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. Results: We d...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2020
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| Online Access: | https://hdl.handle.net/1721.1/125833 |
| _version_ | 1811071884947619840 |
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| author | Friedlander, Jonathan Tsakraklides, Vasiliki Kamineni, Annapurna Greenhagen, Emily H. Consiglio, Andrew L. MacEwen, Kyle Crabtree, Donald V. Afshar, Jonathan Nugent, Rebecca L. Hamilton, Maureen A. Shaw, A. Joe South, Colin R. Stephanopoulos, Gregory Brevnova, Elena E. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Friedlander, Jonathan Tsakraklides, Vasiliki Kamineni, Annapurna Greenhagen, Emily H. Consiglio, Andrew L. MacEwen, Kyle Crabtree, Donald V. Afshar, Jonathan Nugent, Rebecca L. Hamilton, Maureen A. Shaw, A. Joe South, Colin R. Stephanopoulos, Gregory Brevnova, Elena E. |
| author_sort | Friedlander, Jonathan |
| collection | MIT |
| description | Background: Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. Results: We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme from Rhodosporidium toruloides, a heterlogous DGA2 enzyme from Claviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h. Conclusions: The yields, productivities, and titers reported in this study may further support the applied goal of cost effective, large -scale microbial lipid production for use as biofuels and biochemicals. Keywords: Yarrowia lipolytica, Lipid accumulation, Oleaginous yeast, Metabolic engineering |
| first_indexed | 2024-09-23T08:57:30Z |
| format | Article |
| id | mit-1721.1/125833 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:57:30Z |
| publishDate | 2020 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1258332022-09-30T12:25:29Z Engineering of a high lipid producing Yarrowia lipolytica strain Friedlander, Jonathan Tsakraklides, Vasiliki Kamineni, Annapurna Greenhagen, Emily H. Consiglio, Andrew L. MacEwen, Kyle Crabtree, Donald V. Afshar, Jonathan Nugent, Rebecca L. Hamilton, Maureen A. Shaw, A. Joe South, Colin R. Stephanopoulos, Gregory Brevnova, Elena E. Massachusetts Institute of Technology. Department of Chemical Engineering Background: Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. Results: We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme from Rhodosporidium toruloides, a heterlogous DGA2 enzyme from Claviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h. Conclusions: The yields, productivities, and titers reported in this study may further support the applied goal of cost effective, large -scale microbial lipid production for use as biofuels and biochemicals. Keywords: Yarrowia lipolytica, Lipid accumulation, Oleaginous yeast, Metabolic engineering Novogy, Inc. 2020-06-17T14:09:51Z 2020-06-17T14:09:51Z 2016-03 2015-12 2019-09-12T12:28:24Z Article http://purl.org/eprint/type/JournalArticle 1754-6834 https://hdl.handle.net/1721.1/125833 Friedlander, Jonathan, Vasiliki Tsakraklides, Annapurna Kamineni et al. "Engineering of a high lipid producing Yarrowia lipolytica strain" Biotechnology for Biofuels, 9,77 (March 2016): p. 1-12. © 2016 Friedlander et al. en https://dx.doi.org/10.1186/S13068-016-0492-3 Biotechnology for Biofuels Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Nature BioMed Central (BMC) |
| spellingShingle | Friedlander, Jonathan Tsakraklides, Vasiliki Kamineni, Annapurna Greenhagen, Emily H. Consiglio, Andrew L. MacEwen, Kyle Crabtree, Donald V. Afshar, Jonathan Nugent, Rebecca L. Hamilton, Maureen A. Shaw, A. Joe South, Colin R. Stephanopoulos, Gregory Brevnova, Elena E. Engineering of a high lipid producing Yarrowia lipolytica strain |
| title | Engineering of a high lipid producing Yarrowia lipolytica strain |
| title_full | Engineering of a high lipid producing Yarrowia lipolytica strain |
| title_fullStr | Engineering of a high lipid producing Yarrowia lipolytica strain |
| title_full_unstemmed | Engineering of a high lipid producing Yarrowia lipolytica strain |
| title_short | Engineering of a high lipid producing Yarrowia lipolytica strain |
| title_sort | engineering of a high lipid producing yarrowia lipolytica strain |
| url | https://hdl.handle.net/1721.1/125833 |
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