One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination...
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Rockefeller University Press
2020
|
| Online Access: | https://hdl.handle.net/1721.1/125966 |
| _version_ | 1811070767959375872 |
|---|---|
| author | Jacobsen, Johanne T. Mesin, Luka Markoulaki, Styliani Schiepers, Ariën Cavazzoni, Cecília B. Bousbaine, Djenet Jaenisch, Rudolf Victora, Gabriel D. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Jacobsen, Johanne T. Mesin, Luka Markoulaki, Styliani Schiepers, Ariën Cavazzoni, Cecília B. Bousbaine, Djenet Jaenisch, Rudolf Victora, Gabriel D. |
| author_sort | Jacobsen, Johanne T. |
| collection | MIT |
| description | We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation. |
| first_indexed | 2024-09-23T08:41:15Z |
| format | Article |
| id | mit-1721.1/125966 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:41:15Z |
| publishDate | 2020 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | mit-1721.1/1259662022-09-23T13:52:08Z One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation Jacobsen, Johanne T. Mesin, Luka Markoulaki, Styliani Schiepers, Ariën Cavazzoni, Cecília B. Bousbaine, Djenet Jaenisch, Rudolf Victora, Gabriel D. Massachusetts Institute of Technology. Department of Biology We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation. National Institute of Allergy and Infectious Diseases (Grant R01AI119006) National Institute of Allergy and Infectious Diseases (Grant R21AI138020) 2020-06-23T21:22:27Z 2020-06-23T21:22:27Z 2018-09 2018-07 2019-12-10T19:38:54Z Article http://purl.org/eprint/type/JournalArticle 0022-1007 1540-9538 https://hdl.handle.net/1721.1/125966 Jacobsen, Johanne T. et al. "One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation." Journal of Experimental Medicine 215, 10 (September 2018): 2686–2695 © 2018 Jacobsen et al en http://dx.doi.org/10.1084/jem.20172064 Journal of Experimental Medicine Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Rockefeller University Press Rockefeller University Press |
| spellingShingle | Jacobsen, Johanne T. Mesin, Luka Markoulaki, Styliani Schiepers, Ariën Cavazzoni, Cecília B. Bousbaine, Djenet Jaenisch, Rudolf Victora, Gabriel D. One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation |
| title | One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation |
| title_full | One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation |
| title_fullStr | One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation |
| title_full_unstemmed | One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation |
| title_short | One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation |
| title_sort | one step generation of monoclonal b cell receptor mice capable of isotype switching and somatic hypermutation |
| url | https://hdl.handle.net/1721.1/125966 |
| work_keys_str_mv | AT jacobsenjohannet onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT mesinluka onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT markoulakistyliani onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT schiepersarien onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT cavazzoniceciliab onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT bousbainedjenet onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT jaenischrudolf onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation AT victoragabrield onestepgenerationofmonoclonalbcellreceptormicecapableofisotypeswitchingandsomatichypermutation |