One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination...

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Bibliographic Details
Main Authors: Jacobsen, Johanne T., Mesin, Luka, Markoulaki, Styliani, Schiepers, Ariën, Cavazzoni, Cecília B., Bousbaine, Djenet, Jaenisch, Rudolf, Victora, Gabriel D.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:English
Published: Rockefeller University Press 2020
Online Access:https://hdl.handle.net/1721.1/125966

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