Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox‐Based Sensors
Defining perturbations in protein kinase activity within biological samples can provide insight into disease mechanisms as well as potential targets for drug development. In this article, we present a method that utilizes a phosphorylation-sensitive amino acid, termed CSox, to afford kinase-selectiv...
Main Authors: | Beck, Jon R., Peterson, Laura B, Imperiali, Barbara, Stains, Cliff I. |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | English |
Published: |
Wiley
2020
|
Online Access: | https://hdl.handle.net/1721.1/126000 |
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