The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components
GTPase signal transduction pathways control cellular decision making by integrating multiple cellular events into a single signal. The Mitotic Exit Network (MEN), a Ras-like GTPase signaling pathway, integrates spatial and temporal cues to ensure that cytokinesis only occurs after the genome has par...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications, Ltd
2020
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| Online Access: | https://hdl.handle.net/1721.1/126157 |
| _version_ | 1826210514776096768 |
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| author | Zhou, Xiaoxue Amon, Angelika B Campbell, Ian Winsten. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Zhou, Xiaoxue Amon, Angelika B Campbell, Ian Winsten. |
| author_sort | Zhou, Xiaoxue |
| collection | MIT |
| description | GTPase signal transduction pathways control cellular decision making by integrating multiple cellular events into a single signal. The Mitotic Exit Network (MEN), a Ras-like GTPase signaling pathway, integrates spatial and temporal cues to ensure that cytokinesis only occurs after the genome has partitioned between mother and daughter cells during anaphase. Here we show that signal integration does not occur at a single step of the pathway. Rather, sequential components of the pathway are controlled in series by different signals. The spatial signal, nuclear position, regulates the MEN GTPase Tem1. The temporal signal, commencement of anaphase, is mediated by mitotic cyclin-dependent kinase (CDK) phosphorylation of the GTPase's downstream kinases. We propose that integrating multiple signals through sequential steps in the GTPase pathway represents a generalizable principle in GTPase signaling and explains why intracellular signal transmission is a multi-step process. Serial signal integration rather than signal amplification makes multi-step signal transduction necessary. |
| first_indexed | 2024-09-23T14:50:54Z |
| format | Article |
| id | mit-1721.1/126157 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:50:54Z |
| publishDate | 2020 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1261572022-10-01T22:55:41Z The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components Zhou, Xiaoxue Amon, Angelika B Campbell, Ian Winsten. Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT GTPase signal transduction pathways control cellular decision making by integrating multiple cellular events into a single signal. The Mitotic Exit Network (MEN), a Ras-like GTPase signaling pathway, integrates spatial and temporal cues to ensure that cytokinesis only occurs after the genome has partitioned between mother and daughter cells during anaphase. Here we show that signal integration does not occur at a single step of the pathway. Rather, sequential components of the pathway are controlled in series by different signals. The spatial signal, nuclear position, regulates the MEN GTPase Tem1. The temporal signal, commencement of anaphase, is mediated by mitotic cyclin-dependent kinase (CDK) phosphorylation of the GTPase's downstream kinases. We propose that integrating multiple signals through sequential steps in the GTPase pathway represents a generalizable principle in GTPase signaling and explains why intracellular signal transmission is a multi-step process. Serial signal integration rather than signal amplification makes multi-step signal transduction necessary. Eunice Kennedy Shriver National Institute of Child Health and Human Development (U.S.) (Grant HD085866) 2020-07-13T17:46:18Z 2020-07-13T17:46:18Z 2019-01 2019-11-26T15:35:57Z Article http://purl.org/eprint/type/JournalArticle 1534-4983 https://hdl.handle.net/1721.1/126157 Campbell, Ian Winsten, Xiaoxue Zhou and Angelika Amon. “The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components.” , vol. 8, 2019, e41139 © 2019 The Author(s) en 10.7554/ELIFE.41139 eLife Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Zhou, Xiaoxue Amon, Angelika B Campbell, Ian Winsten. The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components |
| title | The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components |
| title_full | The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components |
| title_fullStr | The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components |
| title_full_unstemmed | The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components |
| title_short | The Mitotic Exit Network integrates temporal and spatial signals by distributing regulation across multiple components |
| title_sort | mitotic exit network integrates temporal and spatial signals by distributing regulation across multiple components |
| url | https://hdl.handle.net/1721.1/126157 |
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